Testing efficacy of anaerobic soil disinfestation (ASD) in managing bacterial wilt disease of Hawaiian ginger, turmeric, and tomato crops

Project Overview

GW20-209
Project Type: Graduate Student
Funds awarded in 2020: $25,000.00
Projected End Date: 09/30/2021
Grant Recipient: University of California, Davis
Region: Western
State: California
Graduate Student:
Major Professor:
Tiffany Lowe-Power
University of California, Davis
Major Professor:
Sharon Motomura-Wages
Univerity of Hawaii
Dr. Mohammad Arif
University of Hawaii
Jonathan Beutler
University of California, Davis
Dr. Jonathan Jacobs, Ph.D.
Ohio State University
Dr. Amisha Poret-Peterson, Ph.D.
USDA Agricultural Research Service

Commodities

  • Vegetables: tomatoes
  • Additional Plants: ginger, other

Practices

  • Crop Production: other
  • Education and Training: extension
  • Pest Management: competition, cultural control, disease vectors, eradication, integrated pest management, mulching - plastic, other, prevention, soil solarization
  • Production Systems: agroecosystems, organic agriculture
  • Soil Management: organic matter, other, soil chemistry, soil microbiology
  • Sustainable Communities: other, quality of life

    Proposal abstract:

    This applied crop disease research project is designed to evaluate a novel solution for farmers struggling with bacterial wilt diseases caused by Ralsonia solanacearum. This pathogen damages a remarkably broad range of crops. In the Western SARE region, R. solanacearum is particularly harmful to Zingiberaceae crops in Hawaii such as ginger (Zingiber officinale) and turmeric (Curcuma longa). Outbreaks in ginger spread quickly. They often lead to total losses in fields exposed to the pathogen (Yu 2003), which can remain indefinitely in the soil without effective means of remediation. Because of this, farmers are forced to frequently move their operations, sometimes even carrying the disease along with them on unsanitary equipment or in latently infected seed rhizome (Nelson 2013).

    This research will test an organic management approach called anaerobic soil disinfestation (ASD) for suppressing diverse strains of R. solanacearum, including isolates pathogenic to ginger. Recent studies have shown ASD effectively suppresses strains infectious to other crops. A hopeful example is potato brown rot, caused by a distinct phylogeny of R.solanacearum, exhibiting remarkable suppression through ASD (Messiha 2007). This project will simulate ASD field treatments in soil mesocosms inoculated with diverse global strains for post treatment analysis of pathogen survival and virulence to susceptible host plants. The approach will determine whether ASD could be an effective management strategy for farmers in the Western region.

    Research findings will be disseminated to growers through a combination of public presentations to farmer organizations, print and online publications, and through partnership with Hawaii Cooperative Extension.

    Project objectives from proposal:

    This project will determine whether ASD is an effective management approach against diverse R. solanacearum phylogeny, and it will also examine the taxonomy of R. solanacearum strains that infect Hawaiian turmeric. 

     

    Objective 1: Compare virulence of R. solanacearum wild-type ginger isolates with diverse global strains on model host plants

    Use a controlled experimental approach to test the hypothesis that wild-type ginger isolates are also pathogenic on tomatoes, a practical model host and bioindicator plant, and establish baseline virulence data through observation of wilt symptoms after soil soak inoculations.

     

    Objective 2: Examine R. solanacearum soil population density and virulence responses to ASD treatment amongst diverse natural isolates, including a virulent wild-type ginger strain.

    Use controlled experimental approaches to test the hypotheses that ASD treatment will cause reductions in R. solanacearum soil bacterial concentrations and will also reduce wilt symptoms observed in tomatoes transplanted into post treatment soil as compared to controls.

     

    Objective 3: Identify the taxonomic position of R. solanacearum from infected turmeric (Curcuma longa) plants in Hawaii.

    Use DNA-based taxonomy to characterize the phylogeny of R. solanacearum isolates collected from infested Hawaiian turmeric crops and soil sampled from infested croplands in relation to known ginger strains.

    Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture or SARE.