Soil bacterial diversity responds to long-term establishment of perennial legumes in warm-season grassland at two soil depths

Project Type: Graduate Student
Funds awarded in 2022: $16,454.00
Projected End Date: 08/31/2024
Grant Recipient: University of Florida
Region: Southern
State: Florida
Graduate Student:
Major Professor:
Dr. Hui-Ling Liao
University of Florida
The introduction of rhizoma peanut (RP Arachis glabrata Benth) into bahiagrass (Paspalum notatum Flüggé) may require time to develop stable plant–soil microbe interactions as the microbial legacy of the previous plant community may be long-lasting. A previous study showed that 8 years) into bahiagrass on soil bacterial diversity and community composition against their monocultures at 0 to 15 and 15 to 30 cm soil depths using next-generation sequencing to target bacterial 16S V3–V4 regions. We observed that a well-established RP–bahiagrass mixed stand led to a 36% increase in bacterial alpha diversity compared to the bahiagrass monoculture. There was a shift from a soil bacterial community dominated by Proteobacteria (~26%) reported in other bahiagrass and rhizoma peanut studies to a soil bacterial community dominated by Firmicutes (39%) in our study. The relative abundance of the bacterial genus Crossiella, known for its antimicrobial traits, was enhanced in the presence of RP. Differences in soil bacterial diversity and community composition were substantial between 0 to 15 and 15 to 30 cm soil layers, with N2-fixing bacteria belonging to the phylum Proteobacteria concentrated in 0 to 15 cm. Introducing RP into bahiagrass pastures is a highly sustainable alternative to mineral N fertilizer inputs. Our results provide evidence that this system also promotes greater soil microbial diversity and is associated with unique taxa that require
further study to better understand their contributions to healthy pastures.
Peer-reviewed Journal Article
Adesuwa Erhunmwunse, University of Florida
Victor Guerra, University of Florida
Jung-Chen Liu, University of Florida
Cheryl Mackowiak, University of Florida
Ann Blount, University of Florida
José Dubeux, University of Florida
Hui-Ling Liao, University of Florida
Target audiences:
Farmers/Ranchers; Educators; Researchers; Consumers
Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture or SARE.