Early Leaf Removal Strategies for Bunch Rot Reduction in Pinot Noir Clones

Final report for ONC19-061

Project Type: Partnership
Funds awarded in 2019: $39,977.00
Projected End Date: 12/31/2022
Grant Recipient: Ohio State University-Ashtabula ARS
Region: North Central
State: Ohio
Project Coordinator:
Andrew Kirk
Ohio State University-Ashtabula ARS
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Project Information

Summary:

Pinot noir is currently the highest value wine grape variety in the Eastern US and is among the top cultivated varieties in Ohio (NY Department of Agriculture & Markets 2018, USDA 2017). The variety, however, is known for tight clusters and susceptibility to late-season bunch rot diseases. Recent work has found pre-bloom early leaf removal (ELR) to reduce cluster compactness in tight clustered varieties, while reducing incidence and severity of bunch rot (Acimovic et al. 2017, Hed and Centinari 2018). However, the effects of ELR are variable and highly dependent on variety and localized environmental conditions (Hickey and Wolf 2018). Currently, ELR is considered an experimental practice with little commercial adoption in Ohio.

This proposal sought to introduce ELR to wine grape producers in Ohio, as well as to further understand the physiological impacts of ELR. One major focus of the research effort will be on validating mechanical leaf removal as a cost-effective means to achieve the benefits of ELR treatments. Additionally, this project addressed the diversity of trait expression within the Pinot Noir variety by determining the impacts of ELR on widely planted varietal clones. Importantly, this proposal prioritizes the creation of extension resources to guide ELR adoption in Ohio.

The project set up research projects at three locations, including the Ashtabula Agricultural Research Station and two commercial locations. At the Ashtabula Agricultural Research Station, a trial examined the effect of ELR on the Mariafeld, Pommard, Dijon #115, and Foundation Plant Materials Service #29 clones of Pinot Noir. ELR Treatments were applied in 2019 and 2020 at trace bloom and a selection of data was collected throughout the ripening season of each year. These measurements surveyed rot levels, fruit set metrics, berry counts, yield, canopy density, and juice chemistry , among other parameters. In 2019 and 2020, the Mariafeld clone was particularly sensitive to ELR treatment application, evidenced by fruit set and berry count data. The effect of ELR and the other clones in question was less consistent between years and across various metrics. 

A clonal study was also conducted in 2019 and 2020 at a commercial vineyard site in the Grand River Valley region of Ohio. Here, the research team examined the clones Mariafeld, Dijon 777, and 115 clones of Pinot Noir. Similarly to the work conducted at AARS, ELR was applied manually at trace bloom and the effects were studied throughout the ripening period. Both the Mariafeld and Clone 777 responded to the treatment with improvements fruit quality metrics such as brix and total phenolics.   The application of ELR treatments resulted in a statistically significant reduction in bunch rot across all three clones. However, all three treatments experienced a commercially significant amount of rot, despite ELR treatment, affirming that ELR is not a standalone solution to bunch rot or a replacement for targeted sprays.

At a second commercial vineyard site in the Grand River Valley, a trial was conducted in 2019 to compare the efficacy of mechanical and manual leaf removal. Manual ELR was applied at trace bloom, as was defoliation with a Collard E2200 leaf removal machine. Data was collected to determine whether there were any effects in yield, vine performance, or fruit chemistry when applying ELR mechanically, rather than manually. Across the suite of metrics, numbering more than 10, no statistically significant (p<0.05) differences were observed between the efficacy of mechanical and manual ELR. This finding suggested the mechanical ELR was an acceptable substitute for manual ELR and that financial and practical, rather than horticultural, considerations should drive that investment decision.

Generally, our project concluded that ELR is a helpful practice for vinifera wine grapes in Ohio and similar climates. It did not provide control of bunch rot in our trials, but when used in conjunction with an effective spray program, it is thought to be additive towards that goal. The application of ELR did have positive effects on fruit chemistry in some clones, though the effect was inconsistent. Mechanical ELR was drastically faster than manual ELR and did not produce any measurable negative effects, in comparison to manual ELR. More work is needed still on whether or how to integrate ELR in scenarios where vines have experienced recent spring or winter cold injury.

Project Objectives:
  1. Provide best practice guidance to Ohio vineyards on the implementation of pre-bloom early leaf removal (ELR) as a management strategy for late season bunch rot diseases.
    1. Our project evaluated this strategy and found it to be inadequate as a standalone measure in preventing sour rot and, to an extent, botrytis bunch rot.
  2. Determine the suitability of mechanization for pre-bloom ELR application in Pinot Noir.
    1. Mechanical ELR was found to be a suitable alternative to manual ELR.
  3. Identify sensitivity differences to ELR application among popular Pinot Noir clones.
    1. Of the clones evaluated, the Mariafeld clone of Pinot Noir was found to be sensitive to ELR treatment application.
    2. Other clones (Pommard, 115, 777, 29) exhibited a mixed response to ELR treatment application.
  4. Produce cost-benefit analysis of manual and mechanical ELR application methods.
    1. Mechanical ELR was found to be approximately 60x faster than manual ELR.
    2. Our estimates hold that conversion from manual ELR to mechanical ELR will have a positive ROI after approximately the 250th acre serviced.

Cooperators

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  • Aaron Jaskiewicz (Researcher)
  • Patrick Turner (Researcher)

Research

Materials and methods:

Ohio State University personnel set up randomized experiments at the Ashtabula Agricultural Research Station, St. Joseph Vineyard, and M Cellars in 2019 and 2020. At the Ashtabula Agricultural Research Station and St. Joseph Vineyard, these trials were designed to test whether different clones of Pinot Noir are more sensitive to the bunch architecture changes associated with Early Leaf Removal. At M Cellars, the trial examined the efficacy of mechanical implementation of Early Leaf Removal (ELR), compared to manual application. 

To determine the efficacy of these treatments, OSU personnel collected a wide range of data. At bloom, flower counts were taken for target vines for later determination of the percentage of flowers set to berries. Additional information was collected at this time to support the visual estimation of flowers during bloom. At harvest, fruit chemistry data was collected in addition to berry counts for each target cluster. This will facilitate calculation of percent fruit set, whereas fruit chemistry data will highlight possible changes in fruit quality attributable to the experimental treatment. Yield data at harvest was also collected to assist in the economic analysis associated with implementing the new practice. The team collected and froze berries from the various treatment groups over the course of several years to facilitate wintertime juice analyses on parameters such as brix, pH, Titratable Acidity (TA), and Total Phenolics.

Research results and discussion:

A side by side comparison of mechanical versus manual ELR was conducted at a local vineyard in Harpersfield Twp., Ohio. Perhaps most importantly,  mechanical ELR was drastically quicker to apply than the manual application of ELR. AARS crew averaged roughly 2 minutes per vine to apply ELR manually. Our calculations show Mechanical ELR application to require roughly 2 seconds, per vine, making it approximately 60 times faster than manual application. Our data did not demonstrate a statistically significant difference in the sunlight penetration or remaining leaf area produced by the various treatments, which suggests that the treatment applications produced a similar level of the intended defoliation. The treatments did not produce significantly different Titratable Acidity or Total Phenolics in 2019. In 2019, we found a weak tendency towards higher brix in the manual defoilation treatment (p<0.10). Generally, the conclusion of this project on the question of mechanical vs. manual ELR was that mechanical ELR is an adequate replacement, and is substantially more time efficient. With the two methods being relatively equal in effect, our conclusion was that the decision whether to purchase the equipment involved should focus more on financial considerations, such as the ability to meet the capital outlay, the size and productivity of the vineyard as it relates to potential return on investment, as well as other factors.

Juice Chemistry Analysis: Mechanical vs Manual ELR

 

 2019 Pinot Noir

Brix

Titratable Acidity

(g/L)

Total Phenolics

Mg/L

(gallic acid equivalents)

Control

(Manual)

Treatment

(Mechanical)

Control

(Manual)

Treatment

(Mechanical)

Control

(Manual)

Treatment

(Mechanical)

Mechanical Trial

21.6

20.9*

6.6

6.6

1327

1130

*statistically significant at (p<0.10)

The other two trials in this project related to the efficacy of ELR, applied manually, in various clones of Pinot Noir. One trial was conducted at the Ashtabula Agricultural Research Station (AARS), involving the following clones: Mariafeld, Pommard, Clone 29, and Clone 115. The impetus for this trial was the observation, over numerous years, as to the unique characteristics of the Mariafeld clone found in that vineyard. Typically, we observe that the Mariafeld Clone has significantly looser clusters, with fewer berries per cluster, than other Pinot Noir clones. Data collected in this trial indicated that not only were both of those points true in general, but also that Mariafeld tended to have a higher level of response to ELR treatment.

With regards to berry counts, both Mariafeld and Clone 115 had significantly fewer berries (p<0.05) in the ELR treatment groups, compared to the controls. The other two clones did not exhibit a significant difference in berry count between the ELR and non-ELR groups. Mariafeld and Clone 115 also had significantly lower yield in their ELR groups (p<0.05), which likely related to the discrepancies in berry count between treatment and control. Only the Mariafeld and Pommard clones had a significant difference in the level of rot found in its control and treatment groups. At this site, the rot level was relatively low across all clones. The treatment group of the Mariafeld clone was found to have higher brix, lower Titratable Acidity, and higher total phenolics than the Mariafeld non-ELR control group. The treatment group of the Pommard clone had higher brix than its control, but did not differ on other juice quality metrics. Juice quality differences in the other clones did not pass a test for statistical significance.

Juice Chemistry in ELR Treatment and Non-ELR Control Groups

2019 Pinot Noir 

 

Brix

Titratable Acidity

(g/L)

Total Phenolics

Mg/L

(gallic acid equivalents)

Control

Treatment

Control

Treatment

Control

Treatment

Mariafeld

18.8

20.1*

8.1

7.5*

486

672*

Clone 115

17.8

19.1

6.9

6.4

590

786

Clone 29

17.4

18.4

8.3

7.5

379

339

Pommard

16.9

19.7*

6.2

5.6

312

316

*indicates statistically significant difference (p>0.05)

At a local commercial vineyard site, a similar trial was conducted with the Mariafeld, Dijon 777, and Dijon 115 clone of Pinot Noir. Berry counts per cluster for all three clones were significantly lower in the ELR treatment groups, compared to the non-treatment group (p<0.05). The juice chemistry was positively affected by the ELR treatment group, across all three clones, meaning that brix were higher, TAs were lower, and Total Phenolics were higher in the treatment group. Average yields tended to be numerically lower for the treatment groups, though only that for Clone 115 passed the threshold for statistical significance (p<0.05). The level of rot observed in the treatment groups was significantly lower than the non-ELR control groups. However, even the ELR treatment groups sustained a high level of rot at this site, rendering any statistical differences there mostly irrelevant in terms of outcome to the grower.

Yield Metrics for ELR Treatment and Non-ELR Control Groups

 

 

Yield

(lbs)

Cluster Ct

Weight Per Cluster

(lbs)

Control

Treatment

Control

Treatment

Control

Treatment

Mariafeld

8.9

8.3

32

35

0.27

0.24

777

10.0

8.0

33.8

31.3

0.31

0.28

115

9.5

6.6*

31

25

0.30

0.28

*indicates statistically significant difference (p<0.05)

 

Generally, this project reached several clear conclusions about the role of ELR in Pinot Noir, most of which echo those existing elsewhere in scientific literature. We found that at both sites, application of ELR tended to produce positive effects in juice quality metrics such as brix, Titratable Acidity, and Total Phenolics. Our results indicate that it can have a positive contribution towards controlling rot, but as demonstrated at the aforementioned commercial site, ELR does not necessarily function as a substitute for other measures in controlling rot. Elsewhere, literature differentiates between the improved ability of ELR in reducing botrytis and fungal bunch rots, in contrast to the yeast, bacterial, insect-driven case of Sour Rot. With regards to clonal differences, our results provide compelling evidence that the Mariafeld clone is particularly sensitive to ELR, in addition to having naturally looser clusters in general. Other clones exhibited more inconsistent patterns of relative response to ELR. Interestingly, the Mariafeld clone did perform somewhat differently at the two sites. Our team is in contact with several nurseries to identify whether a particularly loose strain of Mariafeld may be found in the AARS planting.

Participation Summary
3 Farmers participating in research

Educational & Outreach Activities

20 Consultations
15 On-farm demonstrations
2 Online trainings
2 Published press articles, newsletters
4 Webinars / talks / presentations
1 Workshop field days

Participation Summary:

50 Farmers participated
4 Ag professionals participated
Education/outreach description:

Project personnel provided multiple on-farm demonstrations regarding the implementation of Early Leaf Removal. In addition to these efforts, a workshop was held in June 2019. At the Ohio Grape and Wine Conference in February 2020, Early Leaf Removal was a central theme of the technical viticulture sessions, wherein Dr. Maria Smith provided the Ohio Grape Industry with guidance based on our project and the findings of other recent studies. In 2020, Mr. Kirk and Dr. Smith conducted numerous consultations with growers on early leaf removal. Many growers in the grape growing regions of Ohio have adopted this practice. Mr. Kirk presented a summary of this ongoing work at the 2022 Ohio Grape and Wine Conference.

Learning Outcomes

90 Farmers reported changes in knowledge, attitudes, skills and/or awareness as a result of their participation
Key changes:
  • Awareness of potential for reduction of bunch rot severity

  • Awareness of benefits to fruit quality

Project Outcomes

4 Farmers changed or adopted a practice
1 New working collaboration
Project outcomes:

As of March 2023, numerous local grape growers have adopted early leaf removal as a cultural practice to improve fruit quality, lower incidence of disease, and reduce pesticide application. Based on initial surveying, we conclude that 7 local operations have adopted this practice thus far. A workshop on this topic was held in June 2019, which 17 growers attended. 

Success stories:

A grower in Geneva, Ohio mechanized this practice and was able to receive the benefits of early leaf removal in a very cost effective manner. After a severe rain storm in September 2019, his grapes were resilient, possibly in part due to the improvements to skin thickness known to occur after implementation of ELR. A co-operating grower in Madison, Ohio implemented this practice in some, but not all, of his Pinot Noir vineyards. He noticed a marked improvement in the harvest condition of that fruit which had undergone the Early Leaf Removal treatment.

Several large growers in the area have adopted this practice. These growers make up nearly one third of the grape acreage in Ohio. This was encouraging to the project team, as the growers in question had heard about ELR as a practice for many years, but were swayed by the public demonstrations and, importantly, by the commercial growers in the study who adopted the practice and were pleased with results. 

Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture or SARE.