Progress report for SW20-912
Standard almond harvest in California utilizes an on-ground approach which requires multiple machinery passes to shake trees, sweep nuts into row alleys after drying, and collect the crop. This relies on fruit contact with a bare orchard floor, limiting application of organic matter amendments (OMA). This high protein crop requires high nutrient inputs. Inefficient fertilizer use can lead to nutrient leaching, groundwater contamination, and financial losses. Almond huller/processors face management decisions concerning increasing hull and shell biomass. Harvest machinery can disturb topsoil and create dust. Accessible strategies are needed to improve soil health, optimize crop nutrient use, mitigate environmental issues, and optimize hull and shell value.
This project addresses the need for advanced nutrient and harvest management through orchard trials using food-safe OMA to improve soil health and provide nutrients. This creative solution will produce results that guide recommendations using hulls and shells as OMA (HS OMA) to address critical issues currently limiting orchard sustainability. This research will examine complementary off-ground catch frame harvesters to minimize soil disturbance and promote nutrient mineralization.
Research questions will investigate effects of HS OMA management strategies on soil health, plant nutrition, economic value, and environmental impacts:
1. How does almond HS OMA application affect components of soil health?
2. How does HS OMA decomposition and nutrient mineralization impact plant-available nutrients, crop nutrition, and yield?
3. What are the nutrient values, application costs, and associated potential yield effects from HS OMA in different production contexts?
4. What are the broad environmental implications of HS OMA practices?
Research components will utilize randomized complete block design (RCBD) at three sites to evaluate the effects of HS OMA. At one site, a split-plot design will be added to RCBD treatments to compare catch frame harvest to conventional on-ground harvest. Each site will be a case study, independent in location and distinct in design. Outreach and collaboration will share findings among growers, crop advisors, Extension educators, and the public. At on-site field days, growers will discuss practices and guide orchard walk-throughs, prompting dialogue and farmer-to-farmer education. Researchers will present results using posters and engaging take-home materials. A final workshop will integrate feedback and present aggregated data. Researchers will creatively deliver outreach using a decentralized social media approach, engaging existing platforms with partners to provide content through active media outlets such as blog posts, podcast episodes, and videos. At conferences, researchers will present findings and promote social media outlets. Pre/post surveys will assess educational outcomes at events and a stakeholder-developed survey will assess barriers to practice adoption.
This project will promote change in the almond industry by integrating self-generated OMA with nutrient management to improve almond orchard soil health and nutrient management. Assessing benefits and barriers to adoption will ensure research directly assists growers. Results will inform recommendations to improve the sustainability of the almond industry and lay groundwork for future studies. Recommendations will be widely accessible and a variety of management options will allow growers to tailor practices to unique regional contexts.
Soil Health Objective: Assess impacts of almond HS OMA and advanced orchard management strategies on soil health, nutrient mineralization over time, and soil microbial activity related to HS OMA decomposition. Monitor and evaluate changes in soil health measures in three separate case study orchards. Assess broad potential environmental implications of HS OMA and advanced orchard management strategies by evaluating potential changes in soil health and nutrient retention.
Plant Nutrition Objective: Assess the impacts of almond HS OMA and advanced orchard management on almond tree nutrient uptake and crop productivity. Monitor and evaluate HS OMA decomposition over time. Measure changes in crop nutrition by analyzing July leaf samples annually. Analyze annual yield data across treatments. Assess findings in plant nutrient use to characterize crop nutrient uptake from OMA.
Economic Objective: Assess the economic value of HS OMA inputs. Quantify the nutrient value of potassium in HS OMA and compare to potassium content in fertilizer sources. Compare costs of HS OMA practices to current fertilizer costs. Use annual yield data to assess potential economic benefits from HS OMA effects. Document and compare costs of HS OMA and fertilizer application and labor at each site. Document and compare the costs of on-ground and off-ground harvest at Tracy field site.
Educational Objective: Aggregate research data in engaging outreach documents and media that characterizes HS OMA management strategies to facilitate grower adoption. Demonstrate and discuss potential costs and benefits through presentations at conferences, three field days at trial sites, and a final workshop. Prompt discussion among growers and advisors to improve research. Use surveys to identify learning outcomes at all events. During the first year, use an online stakeholder-developed survey to assess potential barriers to adoption for conventional and organic almond growers. Integrate survey findings into outreach activities.
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Research question 1: Can almond hulls and shells be used as a soil amendment over almond tree roots to supply potassium for crop uptake? (Tracy field site)
Hypothesis 1: Surface-applied hulls, shells, and a mix of the two materials can provide mineralized potassium over time for crop uptake. These materials contain different concentrations of potassium and other nutrients and likely release nutrients at different rates. Increased soil exchangeable potassium under amendments could improve tree nutrient status over time as measured in July leaf nutrient values.
Research question 2: How does the application of a hull/shell mix affect soil health and crop performance with and without off-ground harvest in an almond orchard? (Woodland field site)
Hypothesis 2: Microbiological soil health variables most likely to improve under amendments and off-ground harvest (reduced soil disturbance) are increased SOC content, microbial biomass, and a more stable microbial community composition dominated by fungal organisms. Chemical soil health variables most likely to improve are CEC, higher nutrient availability, and potentially pH over time. Soil physical improvements under the combined amendments and off-ground harvest may include improved aggregate stability and water retention by year 3. Crop performance variables most likely to improve under these treatments are improved nutrient uptake, reduced drought stress, and improved crop yield.
Research question 3: What are the effects of almond shell amendments vs. composted shells on soil fertility and water conservation in an almond orchard? Specifically, what are the differences in soil nutrient availability and tree water status over time, and could potential improvements enable savings in nutrient and water inputs? (Davis field site)
Hypothesis 3: The shell amendment will likely provide a mulching effect as a physical barrier on the soil surface to reduce tree water stress particularly during summer months. Shells will mineralize potassium, calcium, and other nutrients and could improve soil organic matter content, CEC, and pH in the long term. The shell-based compost (originally 70% shells, 30% manure) will provide higher amounts of plant essential nutrients which will be reflected in soil and plant nutrient concentrations, but may not provide the same benefits against tree water stress as the shell amendment.
Methods all field sites
- Soil: baseline soil fertility immediately prior to annual applications in the Fall. Soil sampling at 0-10cm, 10-20cm, 20-30cm, and 30-60cm. Analyze for exchangeable potassium, nitrate-N, Olsen phosphate, sodium, calcium, magnesium, CEC, percent soil organic matter, and pH.
- Soil exchangeable potassium in the top 0-10cm at intervals following application.
- Water: ongoing rainfall and evapotranspiration records from the CIMIS database. Ongoing irrigation applied in acre-inches provided by grower records or water meter readings.
- Plant: decomposition rates using mesh litter bags to measure mass loss over time. Percent K remaining in samples of hull/shell materials over time.
- Plant: July leaf nutrient concentrations: sample 20 clusters of 5 leaves per sample tree. 3 sample trees per treatment row.
- Plant: yield across treatment plots including dry kernel lb/ac using weigh wagon, percent crackout.
- Plant: annual trunk circumferences measured in January.
- Plant: stem water potential at key time points in the season to assess drought stress using a pressure chamber.
Additional Methods for Hypothesis 2 (Woodland field site)
- Biomass carbon and nitrogen using fumigation extraction; biodiversity using 16S and ITS was replaced by broad group community composition using PLFA.
- Soil physical characteristics in Fall 2022: aggregate stability using a rainfall simulator, water retention using a Hyprop, and bulk density using a metal ring and mallet.
August 2020-July 2021
Data that is being collected from all field sites will be analyzed this Fall with further results anticipated by the end of 2021. All plans outlined in the original submitted timeline chart have been fulfilled since August 2020 with the exception of implementation of catch frame harvest at the Woodland site last Fall and survey distribution. The catch frame harvest treatment will be implemented in August 2021 and the survey will be distributed at the Almond Conference 2021.
Across all three field sites, the percent potassium remaining in the different hull/shell amendment sources decreased over time, indicating potassium released into soil solution. Corresponding substantial increases in soil exchangeable potassium were found at all three sites, regardless of irrigation type. The potassium solubilization curves from these field sites resemble similar potassium release curves from other crop residues as described in the review paper, Andrews et al. 2021. Ms. Andrews is currently working with a statistician to develop a model for estimating percent potassium released form these amendments as a direct function of water applied (irrigation and rainfall). Potassium release is characterized by a dramatic reduction in K within the first 3 inches of applied water, followed by a more gradual reduction over time. Overall, around 30-50% of initial K was released within the first 3 inches of water, and then around 80-95% of total K was released by the following summer. Generally, from Fall until the following summer, shells decomposed around 20-30% total, the shell-based compost at Bullseye decomposed by around 50%, hull/shell mix decomposed around 50%, hulls decomposed around 60%. When allowed to remain on the soil surface under catch frame harvest, the hull/shell mix at Westwind decomposed by 45% after 365 days.
At Crown Nut Company, there were no statistically significant differences in July leaf values in 2020 or 2021. There were no signs of any potential nitrogen immobilization in the soil impacting July leaf N values. Average leaf K was slightly higher for the amended trees in July 2021, though nonsignificant. Amendments maintained yield in 2020 and 2021 as there were no differences in average dry kernel lb/ac or average % crackout. While a small percentage of amendment materials were found in yield samples, the grower/processor reported this did not interfere with processing. Hulls, mix, and shells released an estimated 103-135 total lb/ac of potassium at approximately $52-68/ac value. The grower reported that potassium sulfate fertilizer costs around $200 for 400 lb/ac, whereas the amendment materials are free for them.
At Bullseye, from Fall 2020 application until the following summer, the shells released approximately 80% of total K while compost released approximately 60%. However, dry mass remaining was approximately 70% for shells and 50% for compost, illustrating that potassium release is not strongly limited by decomposition rate. The shells released a higher fraction of initial potassium, and the compost decomposed relatively more than the shells. Potassium release corresponded with significant increases in soil exchangeable potassium under both amendments from around one month (1.85 inches of water) following application onward. All July leaf nutrients were in the optimum range, and there were no significant differences between treatments. Average leaf potassium under shells was slightly higher, though nonsignificant. Shells and compost maintained yield as there were no significant differences in average dry kernel lb/ac or average percent crackout.
At Westwind, the hull/shell mix amendment applied in Fall 2020 released around 95% of total initial potassium by June. This corresponded with significantly higher soil exchangeable potassium particularly in winter and spring. After one year, the amendment decomposed only by around half of its dry weight. Average July leaf nutrients in 2021 showed statistically significantly higher leaf potassium and lower leaf magnesium under the amendments. However, all macronutrients fell well within the recommended range, except nitrogen which was similarly deficient in both amended and control trees. Stem water potential was significantly less negative for amendment trees when measured six days after irrigation events in April, June, and July, but not when measured only 3 days after irrigation. This suggests that the amendment can reduce tree drought stress particularly during the later period of an irrigation cycle. Collaboration with irrigation scientists using Electrical Resistivity Tomography imaging indicated that the amendment provided a mulching effect by increasing irrigation infiltration rate and reducing evaporation from the soil surface. Average kernel lb/ac yield and average percent crackout were both slightly higher for amended catch frame harvested trees, though not statistically significant. Issues with the catch frame equipment illustrated the importance of working with effective equipment that is adjusted appropriately for almond trees and experienced operators who can ensure efficacy in the future.
At Westwind, baseline microbial biomass carbon in Fall 2020 was similar across all treatment rows. By Fall 2021 we saw significant (p<0.001) treatment effects. Control rows had the least microbial biomass carbon, with the unamended off-ground harvest rows having slightly more. The rows which had received the almond hull and shell amendment had the most microbial biomass carbon, with the amended off-ground harvest rows having even more than their amended on-ground harvest counterparts. Statistical analysis showed that when amendment and harvest type were analyzed separately for Fall 2021, the effect of amendment was highly significant (p<0.0001) while the effect of harvest type was not yet significant (p=0.1486). In the intermediate timepoint, Spring 2021, the reverse order of treatments was observed, with the control rows having the highest microbial biomass carbon and the amended rows having the lowest, though the treatment effect at this timepoint was not statistically significant. This intermediate step is likely the effect of temperature, with the amendment acting as a mulch to increase soil moisture retention and create cooler soil temperatures, especially in the spring. By the fall, however, the hypothesized treatment effects had emerged.
At Westwind, samples were taken for 16S and ITS shotgun sequencing at three time points and stored at -80 C. However, after contracting with a sequencing laboratory, we were told that they were unable to extract enough DNA to move forward with sequencing. Instead, microbial community composition was assessed using PLFA (phospholipid fatty acid analysis) in both the amendment organic layer and the top 0-10cm soil. In October 2021, the 1 year old amendment layer contained around double the microbial biomass compared to the 1 week old amendment layer. The original amendment had higher total bacteria and total fungi biomass, specifically higher actinomycete, saprophyte, mycorrhizae, and protozoan biomass, with a higher ratio of fungi to bacteria. Compared to the control soil, the soil microbial community under the combined amendment and catch frame treatments contained higher average total biomass, including both higher total bacteria and fungi biomass, specifically higher actinomycete, mycorrhizal, and protozoan biomass with a very low fungi to bacteria ratio. The PLFA data indicates that the amendment layer is full of microbial biomass, can support higher trophic levels such as protozoa, and is building a microbial food web over time. Compared to control soil, the soil with the amendment and catch frame contained higher soil microbial biomass after one year and Principal Components Analysis indicated that higher bacterial biomass in particular distinguished it from the control soil. There was a slight increase in arbuscular mycorrhizal biomass under the amendment as well.
Education and Outreach
To date, our almond grower collaborators hosting the three field trials and their respective agricultural consultants have provided feedback about critical areas of crop system management to consider during the creation of the stakeholder-developed survey. They commented on barriers to adoption, motivating factors, and other implementation considerations. Their input has been integrated into the survey draft which will be distributed widely to growers at the Almond Board Conference 2021.
Furthermore, Dr. Khalsa gave a talk on March 18th about soil amendments in orchards with a focus on dairy manure compost. The virtual field day hosted by another UC Davis collaborator and supported by the California Dairy Research Board and the Almond Board of California was attended by 65 participants, approximately 12 farmers and 53 agricultural professionals. Discussion ensued about the use of almond byproducts like hulls and shells and how they are used as dairy feed for the high quality hulls, but also how lower quality shells can be added to dairy manure compost. The synergy between the dairy and almond industires will be an essential partnership for future sustainability of California agriculture.
Education and Outreach Outcomes
- Compost Feedstocks
- Use of almond hulls & shells as organic matter amendments