Progress report for GNE20-233
Crop damage is a persistent issue faced by farmers nationwide, resulting in staggering costs to farmers in crop loss. Ungulate species such as white-tailed deer (Odocoileus virginianus) and sika deer (Cervus nippon) are a major source of agricultural damage through extensive browsing. Often a farmer’s only solution to mitigate browsing losses is through crop damage permits to hunt deer on their farm. However, deer populations occur over larger scales than a single property, limiting farmers’ abilities to actually reduce local deer densities through hunting and leaving them ineffective in reducing crop damage by ungulates. Surrounding habitats can reduce or exacerbate impacts on crop damage – reducing damage by providing additional food that lowers the intensity of deer browsing on crops or exacerbating damage by providing refuge to deer that actively browse on agricultural fields. My research looks to examine both the effect that adjacent habitat composition and configuration has on crop damage as well as quantify the impact that dietary overlap of two co-occurring ungulates has on crop damage. High-resolution dietary analysis using metabarcoding plant DNA present in ungulate fecal pellets allows crop damage to be quantified. Combining the dietary profile of ungulates with landscape level metrics of the surrounding habitats will reveal how habitats in close proximity to agriculture influence crop damage. Through informing famers, policy makers and state agencies about the influence of adjacent habitats on crop damage, this work will develop management polices beyond deer culling to effectively mitigate crop damage.
The specific objectives of this research are to:
(1) Determine the impact that the composition and configuration of neighboring habitats (forests, marshes, fallow fields) within agricultural landscapes have on the proportion of agricultural crops in a deer’s diet.
Hypothesis 1: Overall area, number of patches, and connectivity of neighboring habitats will decrease the proportion of agricultural species present in the diet of the deer.
Hypothesis 2: The proportion of agricultural species present in the diet of the deer can be explained by the composition and configuration of neighboring habitats.
Hypothesis 3: Reliance on plant species present in neighboring habitats will vary through time depending upon the abundance and availability of crops.
(2) Evaluate the impact of dietary niche overlap of sympatric deer species has on crop damage.
Hypothesis 1: We will observe higher overall deer densities in regions where both deer species occur than regions where just white-tailed deer or sika deer occur in isolation.
Hypothesis 2: Agricultural species will make up a smaller proportion of the diet of white-tailed deer and sika deer in regions where they co-occur due to interspecific competition for crop resources.
Hypothesis 3: Despite lower per capita crop consumption, crop damage will be greater in areas where the species co-occur due to higher densities.
Hypothesis 4: Higher deer densities will be supported by a greater reliance on plant species from neighboring habitats, and we expect to see a wider dietary breadth in regions where both species co-occur.
The purpose of this project is to examine the effect that landscape configuration and composition of surrounding habitat types has on crop damage by deer. Crop damage is a persistent issue faced by farmers nationwide; surveys have shown that crop damage inflicted by deer is the most widespread form of wildlife damage to crops (Conover and Decker 2018; Wywialowsky 1994, Conover 1998). Ungulates, specifically white-tailed deer (Odocoileus virginanus) and sika deer (Cervus nippon), damage crops through browsing and are a major source of agricultural damage, both of which occur on the Eastern Shore of Maryland (Claslick and Decker 1979, Conover 2001, Conover and Decker 2018, DeVault et al. 2007, Springer et al. 2013). Often a farmer’s only solution to mitigate crop damage is through crop damage permits that allow the increased harvest of deer on their farms. However, culling deer on-site may not solve the problem, as deer populations occur at larger scales than a single property. Although crop damage by deer often correlates with deer population density, not all deer present in an agricultural landscape consume the same proportion of agricultural species (Bleier et al. 2012). Agricultural fields do not occur in isolation but are surrounded by a complex matrix of habitats. Deer frequently utilize habitats surrounding agricultural fields. How do surrounding habitats affect deer consumption of crops? Surrounding habitats have been suggested to negatively and positively impact the amount of crop damage occurring on the adjacent fields. Deer can forage on plant species present in habitats surrounding agricultural fields mitigating deer browsing on crops, or deer can utilize these habitats as refuge resulting increased deer densities and increased browsing pressure on crops. However, no research to date has tried to directly quantify how surrounding habitats mitigate or intensify crop damage.
My research will utilize a relatively new methodology of DNA metabarcoding of deer fecal pellets to construct of high-resolution diet profiles of two co-occurring ungulate species in agricultural landscapes. The DNA barcoding approach will allow me to identify all plant species, crop and non-crop, and their relative contribution to deer diets. Quantifying crop damage inflicted by the deer present in the agricultural landscape on an individualistic level. Combining the dietary profiles of deer on agricultural fields with landscape metrics such as patch size, patch density, and patch connectivity of adjacent habitats will allow for a better understanding of how these neighboring habitats to agriculture affect crop damage. Data on how adjacent habitats influence crop damage will provide management recommendations to mitigate crop damage for farmers and policy makers. As many agricultural landowners in my study region own adjacent habitats as well as their fields, there will be a high opportunity for implementing deer management in adjacent habitats. Strategic community partners (see letters of support) will help me to develop recommendations for best practices based on my findings and disseminate results to land owners beyond the few sites where the research will be conducted.
The following objectives will be accomplished via field experiments established in Dorchester and Somerset Counties, Maryland and lab work conducted at George Washington University.
Objective 1. Evaluate the impact that the configuration and composition of adjacent habitats to agricultural fields in the landscape have on crop damage.
In order to test how varying composition and configurations of adjacent habitats influence crop damage, I will construct high-resolution diet profiles of deer. High-resolution dietary analysis provides a mechanism to quantify the level of crop damage caused by a single deer. Plant DNA can be extracted from fecal pellets. Using next generation sequencing and metabarcoding techniques, plant material in the fecal pellets can be identified to the species level (Kartzinel et al. 2015). Metabarcoding utilizes short sections of DNA from a plant gene compared against reference libraries of this gene to pinpoint the sequence to a specific species. I will barcode two genes to increase the accuracy of results: 1) the gene for the large subunit of the ribulose-biphosphate carboxylase (rbcL) and 2) the internal transcribed spacer (ITS) gene. The Consortium for the Barcode of Life (CBOL) plant-working group refers to these two genes as “core barcodes” for plant identification. Metabarcoding will allow me to identify the relative frequency of occurrence for each plant species (i.e. presence/absence of a certain plant species averaged across all fecal pellets) as well as the proportion of each species present in the fecal pellet utilizing relative read abundance (RRA) (Kartzinel et al. 2015).
Fecal pellets will be collected randomly throughout the predominant habitats in the landscape (agricultural fields, forested regions, and marshes). Time-based fecal pellet accumulation curves will be utilized to ensure an exhaustive search. The number of fecal pellets discovered will be graphed by the time spent collecting these fecal pellets, making sure that the number of new fecal pellets discovered asymptotes indicating overall fecal abundance. This indicates sampling effort is adequate to collect all fecal pellets present. Fecal pellets will be collected during 4 time points throughout the year in order to understand the seasonal variation in the importance of neighboring habitat. Collections will occur in March prior to planting, June during the growing season of the crops, September-October prior to harvest, and lastly in December after cover crops have been established.
Fecal pellets will be visually inspected for freshness based on pellet color, consistency, and sheen (Brinkman et al. 2010). Fecal pellets will be ranked for freshness ranging from high quality (sticky with mucus, slight sheen on pellets, soft texture) to low quality (firm and dry but still with a sheen of mucus) (Kalb and Bowman 2018). We will not count fecal pellets that show signs of mold, appear broken or decayed, or lack a mucosal sheen on the exterior. A total of 30 fecal pellets will be analyzed for plant DNA per habitat per time point, resulting in over 360 fecal pellets included in dietary analyses. A sample-based species accumulation curve will ensure the number of samples accurately reflects the plant species present in the diet of the deer. Fecal pellets will be randomly sampled from those collected in the field to be included in the diet analysis. We will utilize a fecal pellet index to quantify deer density at each farm sampled (following Forsyth 2005).
A GPS unit will provide an exact location of each fecal sample collected, which will provide the spatial data necessary to conduct landscape analysis. Spatial buffers will be created around the point locations of the 30 fecal pellets included in the dietary analysis. Buffer size will be the average distance traveled by deer in this region (Eyler 2001) multiplied by the food passage rate (Mautz and Petrides 1971), which will approximate the region in which foraging occurred for that fecal pellet. Within buffers, I will measure the landscape variables of patch size (overall area of each habitat), patch density (number of habitat patches), and patch connectivity (links or overlap between similar habitat patches).
In order to evaluate hypothesis 1, I will conduct a multiple regression to estimate the quantity of agricultural species present in the diet of the deer as a function of the season of sampling, deer density, and the calculated landscape metrics as predictor variables. This will allow us to directly access how well the composition and configuration of neighboring habitats predicts crop damage. Hypothesis 2 will be evaluated by Akaike information criterion (AIC) comparing models only using deer density to explain the percentage of agricultural species in their diet with models using landscape metrics. Conducting a multivariate multiple regression to predict the proportion of each species present in the diet of the deer as a function of deer density, season of sampling, and landscape metrics will assess hypothesis 3.
Objective 2. Evaluate the impact of dietary niche overlap of sympatric deer species on crop damage.
To investigate how dietary niche overlap of co-occurring deer species (white-tailed and sika) impacts crop damage, we will take advantage of the disjunct and overlapping spatial distribution of these two species in the Lower Eastern Shore of Maryland. Using many of the same methods as in the previous objective, we will identify the relative frequency of occurrence for each plant species as well as the proportion of each species present in the fecal pellet when the two deer species occur alone or together. As above, metabarcoding of the rbcL and ITS plant genes in fecal pellets will enable us to determine high-resolution diets of both species of deer. However, in addition to the methods described above, I will also sequence DNA of a “minibarcode” of the mitochondrial gene Cytochrome Oxidase 1 (CO1) used to differentiate animal species. I will use this minibarcode sequencing to discern the deer species from which the fecal pellets originated.
Sika deer and white-tailed deer on the Eastern Shore of Maryland occur in isolation and sympatrically. Fecal pellets will be collected throughout regions in which both species occur (Buck Range Farm; see letter of support from Donald Webster), and regions in which they occur in isolation (e.g. Blackwater National Wildlife Refuge for sika deer; see letter of support from Matt Whitbeck) (Somerset County for white-tailed deer; see letter of support from Sarah Hirsch). As in Objective 1, time-based fecal pellet accumulation curves, seasonal sampling, and pellet freshness quality assurance will be used in a rigorous and informative sampling protocol. A total of 30 fecal pellets will be analyzed for plant DNA for each of the 3 regions (only sika, both species, and only white-tailed) per time point in four time points, resulting in 360 fecal pellet samples for dietary analysis. Again, we will utilize a fecal pellet index to quantify deer density at each farm sampled.
Statistically, I will compare whether deer density or deer diet preferences are a stronger predictor of deer crop damage. In order to test Hypothesis 1, a one-way ANOVA will be conducted using total deer density as a response variable and deer species present in the region (whitetail deer, sika deer, or both) as a predictor. To evaluate Hypothesis 2, I will utilize a two-way ANOVA with the quantity of agricultural species present in the diet of the deer as a response variable and predictor variables of season of fecal pellets collection (pre-planting, growing season, pre-harvest, cover crop) and deer species present. This will allow me to test if the amount of agricultural species present in the diet of each deer varies depending upon if they occur in isolation or together. In order to evaluate hypothesis 3, I will calculate total crop damage inflicted by taking deer density at each site and multiplying it by the average percentage of agricultural species present in their diet. We will then conduct a one-way ANOVA using this total crop damage as the response variable and whether the region contains whitetail deer, sika deer, or both species co-occur as a predictor variable. Lastly, I will use non-metric dimensional scaling (NMDS), an ordination technique, to understand how the plant community present in each species’ diet varies and test hypothesis 4. I will use Analysis of Similarity (ANOSIM) to test for differences between each species’ diet and vector fitting to test how deer density affects diet composition.
Initial sampling efforts were conducted in late August to early September 2020 at 2 farm sites (Barneck Farm and Anderson Farm) a total of 17 fecal pellets were collected. A total of 9 fecal pellets were collected at the Anderson Farm, with 3 fecal pellet samples coming from each of the 3 habitat types present on the farm (marsh/fallowed farm fields, agricultural fields, and forest). A total of 8 fecal pellets were collected at the BarNeck Farm, with 5 fecal pellet samples coming from the forest and 3 fecal pellet samples coming from the agricultural fields. Sampling efforts had to be paused from September 15, 2020 - January 31, 2020 due to the hunting season and access to the farms during this time. Sampling efforts will resume at the conclusion of hunting season on these farms, as well as initial sampling occurring at agricultural sites in Blackwater National Wildlife Refuge and other farm sites located in Dorchester and Somerset County, MD. We are currently working with extension agents in Somerset and Dorchester Countries to access addition farm sites experiencing heavy crop damage due to deer. Fecal pellets will be sampled at these sites throughout 2021 to obtain temporal variability in the deer's diet choice. Overall sampling effort will drastically increase in 2021 in the first full year of the grant.
Initial DNA extractions of the 17 fecal pellets sampled in 2020 are currently occurring in our laboratory at George Washington University. DNA "meta-barcoding" of the initial 17 samples will occur in February and March of 2021. Addition DNA extractions and DNA "meta-barcoding" will occur throughout 2021 as addition fecal pellet samples are collected from the field.
Education & Outreach Activities and Participation Summary
This project will leverage the outreach program of a larger project funded by the USDA’s National Institute for Food and Agriculture (NIFA) that is researching the impacts of saltwater intrusion on agricultural fields in the Lower Eastern Shore of Maryland. The NIFA project has annual stakeholder meetings each spring in which farmers, extension agents, and other agricultural professionals participate, and I will have an opportunity to share my research results with this group. University of Maryland Agricultural Extension Agents will also help me to disseminate my findings. Sarah Hirsch in Somerset will assist me in this aim (see letter of support from S. Hirsch). My research will be shared at a state level in collaboration with the Maryland Department of Agriculture, which is responsible for developing Best Management Practices for agriculture. I plan on presenting my findings with Maryland Farm Bureau at their annual meeting in December 2021, a leading voice of Maryland agriculture and predominant force in agriculture policy development in the state. I am in contact with Brian Eyler, the head of the Deer Project for the Maryland Department of Natural Resources, who is directly responsible for the management of deer populations and mitigation of crop damage within the state. Lastly, I will communicate my findings directly to local farmers during farm field days and extension meetings on the Eastern Shore of Maryland after the completion of my project in 2021. Enabling farmers experiencing extensive crop damage due to deer herbivory to make informed decisions on how to mitigate the intensity of crop damage occurring on their farm is the motivation for this research.