Evaluating Entomopathogenic Nematodes for Biological Control of Apple Maggot Fly under Field Conditions

Final report for GNE20-238

Project Type: Graduate Student
Funds awarded in 2020: $14,770.00
Projected End Date: 10/31/2021
Grant Recipient: University of Massachusetts
Region: Northeast
State: Massachusetts
Graduate Student:
Faculty Advisor:
Dr. Jaime Pinero
University of Massachusetts
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Project Information


Evaluating Entomopathogenic Nematodes for Biological Control of Apple Maggot Fly under Field Conditions.

One of the most valuable fruit crops in the USA, apple, is attacked by many pests. The apple maggot fly (AMF), Rhagoletis pomonella (Diptera: Tephritidae) is endemic to eastern North America from Canada via the USA to Mexico (CABI, 2019), and is considered as one of the key insect pests in the Northeast. The pupae (soil-dwelling stage) overwinter under the top 5 cm of soil beneath the apple trees and emerge as a new generation of univoltine adults in late spring. To control R. pomonella, growers typically apply up to three broad-spectrum insecticide sprays to the entire orchard. Foliar sprays target the adult stage. No management options are currently available to kill larvae and pupae of R. pomonella in the soil. Entomopathogenic nematodes (EPNs) have been evaluated as biological agents against many pests. For R. pomonella two EPNs species, Steinernema carpocapsae and Steinernema riobrave, showed promising results and effective candidates under laboratory conditions. The field evaluation results indicated that S. riobrave, as high dose, (= 200 Infective Juveniles (= IJs) per cm2 proved to suppress the immature stages of R. pomonella in the field during fall and winter. Hence, growers would have another integrated pest management (IPM) tool, leading to reductions in insecticide use against R. pomonella supporting more sustainable apple production.

Project Objectives:

The research objective of this project was two-fold:

Laboratory study: Evaluated the virulence of different dosages of the entomopathogenic nematodes (EPN) S. carpocapsae and S. riobrave (high = 200 Infective Juveniles (= IJs) per cm2, and low = 50 IJs per cm2) alone and in combination under laboratory condition using field-collected soil samples.

Field study: Evaluated the virulence of S. riobrave (high = 200 Infective Juveniles (= IJs) per cm2) against R. pomonella pupae in four commercial apple orchards. The previous research by Pinero et al. 2019 with plum curculio (another apple key pest) proved that these two nematodes are highly effective to plum curculio in the apple orchard condition. Therefore, if these two nematodes show to be highly effective for apple maggot fly larvae and pupae, we kill ‘two birds with one stone.



The purpose of my project was to evaluate two promising entomopathogenic nematode (EPNs) species to control larvae and pupae of the apple maggot fly, Rhagoletis pomonella (Diptera: Tephritidae), potentially leading to more sustainable management of this key apple pest. If successful, then growers could reduce insecticide use against R. pomonella because they will have a low-cost biological control tool that is not currently available to control this pest.

Apple is one of the most valuable fruit crops in the USA. In fact, the most recent data in the US value of utilized non-citrus production apple place in the second in the nation with 4.94 billion tones just after grape (USDA NASS, 2019). In New England, Massachusetts ranked first with an average value of utilized production of approximately 31 million /year (USDA NASS, 2019). In eastern North America, apple maggot fly, Rhagoletis pomonella, has been ranked as a key pest of apple. If left uncontrolled, it brings economic damage at about 30-70% or even 91% out of total production (Prokopy et al. 2003). To maintain AMF populations below economic damage, apple growers depend on applying broad-spectrum chemical pesticides to the entire orchard. Inevitably, it negatively impacts the biological community such as beneficial arthropods, including many species of predators and parasitoids that suppress several pests, as well as wild pollinators such as bees and syrphid flies in the orchard ecosystem. Pesticides also influence the functioning of agricultural soils, while their leaching to the surface produces a threat for the provision of the water resource and for the functioning of aquatic systems (Putten et al. 2006).

For many years, apple growers in Massachusetts and other New England states have supported apple IPM. They are likely to adopt ecologically-based IPM tools and strategies if those options are economically feasible and effective. However, in recent years the bulk of research and extension efforts related to fruit pest management has focused on evaluating newer chemistries that could replace the use of broad-spectrum insecticides, and research aimed at developing alternative IPM methods for arthropod pest has decreased substantially. I believe research in the area of biological control of apple pests is needed. A survey of 88 commercial apple growers from Massachusetts and Rhode Island was implemented by Dr. J. C. Piñero (project advisor) in mid-April 2018. Survey results revealed that for 32% of the growers AMF is the most damaging pests in apple orchards. Most growers indicated that more IPM-oriented research is needed.



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  • Dr. Masoud Hashemi (Educator and Researcher)


Materials and methods:

EPNs: The EPN S. riobrave (355 strain) was shipped overnight from the USDA ARS Southeastern Fruit and Tree Nut Research Laboratory in Byron, GA, to UMass Amherst for the field study.

Sources of apple maggot fly: Wild populations were collected originally from field-infested (unsprayed sections) of UMass and Amherst where abandoned apple trees  exist. To get R. pomonella pupae for the experiment, infested fruit was placed in groups of 35 each on top of a wire screen (0.5 x 0.5x m) 5 cm above a 2.5-cm thickness of moist sand, the pupation medium stayed there until developing larvae had formed puparia. The laboratory population (Fig. 1) was provided by the USDA ARS Appalachian Fruit Research Station in Kearneysville, WV.

Laboratory experiments: Two different experimental groups were structured. In the first one (A), the wild population of R. pomonella pupae (as univoltine) had exposure to different dosages of S. carpocapsae and S. riobrave (high dose = 200 Infective Juveniles (= IJs) per cm2, and low dose = 50 IJs per cm2) alone and in combination. The soil source was collected from autoclaved soil under standard conditions for this exposure. Control had no exposure to EPNs but was associated with water.

In the second group (B), EPNs dosage was similar while R. pomonella pupa population and soil type altered, so that the laboratory population of R. pomonella (multivoltine) was exposed to EPNs. The soil was applied from the University of Massachusetts Cold Spring Orchard Research & Education Center, where EPNs were applied to the soil against plum curculio in July 2020. The Control group had no exposure to EPNs and was associated with water. 840 R. pomonella pupae were exposed to EPNs in transparent plastic containers under ambient conditions for both groups. Four replications for each dosage were conducted. All samples were observed during the first 72 hours to record the infestation rate. All samples were kept under ambient conditions to emerge R. pomonella and record the mortality data (Fig. 1).



Figure 1: Illustration of the experimental steps referring evaluation of different EPNs dosages against the different sources of R. pomonella pupae (Wild and laboratory) and different soil types (The soil was applied from the University of Massachusetts Cold Spring Orchard Research & Education Center, where EPNs were applied to the soil against plum curculio in July 2020.) (H; high dose = 200 Infective Juveniles (= IJs) per cm2), (L: low dose = 50 IJs per cm2), (Sr: S. riobrave), and (Sc: S. carpocapsae).


Field experiments: This research was conducted at four different commercial apple orchards in Massachusetts (Red apple orchard in Phillipston, Sentinel farm in Belchertown, University of Massachusetts Cold Spring Orchard Research & Education Center in Belchertown, and Small Ones farm in Amherst). I used a powerful lure (AMF blend) to attract R. pomonella to specific trees. Those trees were not sprayed with insecticides leading to more significant fruit infestation by wild R. pomonella. In September 2020, two areas were marked under each apple tree canopy. Each area (1m2) received 35 wild R. pomonella pupae, which were placed on the top 5 cm of the soil. Then one of the blocks received a high dose of S. riobrave (200 IJ cm2) and the second block just received water as a control. Sixteen experimental units were created across all four orchards. In December 2020, we returned to the blocks to collect all pupae to transfer under laboratory conditions to record R. pomonella adult emergence (Fig. 2).


Figure 2: Illustration of the experimental steps for the fall evaluation of S. riobrave virulence against soil-dwelling stages of R. pomonella in commercial apple orchards. (a) Summer application of R. pomonella pupae under the apple tree canopy, (b) Application of S. riobrave against R. pomonella to the soil; (c) Late fall removal of R. pomonella pupae from the soil.

Data analysis:

Data were analyzed by t-test, ANOVA, GLIMMIX procedure of SAS (version 9.4, SAS Institute, Inc., Cary, NC, Online version), and mean separation performed using Duncan's multiple range test (P < 0.05) to separate.



Research results and discussion:

Laboratory experiments: Dose-Response Experiments

Nematode isolates evaluated by dose-response have shown different infection rates on R. pomonella pupae. Soil type and source of host population showed a significant effect in emergence rates of adult R. pomonella from pupae that received EPN applications. Soil samples that were collected from Cold Spring Orchard, which received applications of EPNs against another pest (plum curculio) showed significant levels of suppression than autoclaved soil (F= 13.75; df=1; P< 0.0005) (Table 1). 

Appraising suppression emergence rate based on different EPNs dosage regarding soil and population source indicated that there was a significant between the different dosages of both groups A (A: Autoclaved soil + laboratory population of R. pomonella) and B (B: Non-autoclaved soil + wild population of R. pomonella) (Table 2).


Appraising suppression emergence rate based on the interaction effect of soil type and different EPN application dosages, indicated that the combination of two EPNs as (S. carpocapsaeS. riobrave) in a high dose in the soil showed the highest suppression of R. pomonella pupae (0.25± 0.25) while control treatment in autoclaved soil showed the lowest suppression power (4.5± 0.95) (F= 3.53; df=13; P< 0.0009) (Table 3).

Apprising suppression based within-group differences (Similar dosage with different soil and population) showed a significant difference on control, combination dosage (S. carpocapsae S. riobrave) high and low respectively (Fig. 3).

Figure 3: Mean emergence rate of R. pomonella with the exposure of the same dosage of EPNs, but different soil types.
*Above bars denote statistically significant differences between treatments according to ANOVA (P= 0.05).


Field experiments: experiments:

Results of the fall application of fly pupae and the EPN S. riobrave under field conditions demonstrated that R. pomonella pupa emergence rate was significantly reduced in the blocks where there was the application of S. riobrave high dose (t = −2.72; df = 14; P = 0.017) (Fig. 4A). This confirms that S. riobrave can markedly reduce the survival rate of R. pomonella pupae to emerge as adults during late fall, under colder soil conditions as those experienced in the summer (Fig. 4B).

Figure 4: A: Mean emergence rate of R. pomonella after pupal exposure to a high dose of S. riobrave (200 IJ cm2) and water as control from September to December 2020;    B: Maximum, minimum, and average soil temperature recorded by the smart agricultural weather station at Cold Spring Orchard, University of Massachusetts Amherst.      *Above bars denote statistically significant differences between treatments according to ANOVA (P= 0.05).
Figure 5: Left to right respectively: S. riobrave activity and survival in soil; penetrating S. riobrave into the pupa; Infected and healthy pupa appearance.


Complementary procedure to confirm results:

Re-isolation of the S. riobrave from infected Galleria mellonella, wax moth (Lepidoptera: Pyralidae) was done to satisfy Koch’s postulates. Twenty healthy 5th instar larvae of G.  mellonella were placed in 9 cm Petri dishes which were lined with different species infective stage EPN (200IJs/dish) filter paper. Cadavers’ larvae with signs of EPN-infection, which is recognized by a change in color (usually ochre/brown/black for Steinernematids), were rinsed in sterile distilled water. Then each individually transferred in modified white traps (Kaya & Stock 1997) for the emergence of the free-living infective stage juvenile nematode. Emerging nematodes were pooled for each sample and used to infect fresh G. mellonella larvae to confirm Koch’s postulates of pathogenicity. Recording larvae mortality indicated of Nematode viability was ≥95% in all experiments 24 h, 48h, and 72h, (F= 6.22; df=1; P< 0.0257), (F= 15.24; df=1; P< 0.0016), 72- (F= 75.85; df=1; P< 0.0001) correspondingly (Fig. 6).

Figure 6: Koch’s postulates results: Cumulative means mortality rate of G. mellonella after the exposure to high dose (H) of S. riobrave.
*Above bars denote statistically significant differences between treatments according to ANOVA (P= 0.05).

Additional data: During our procedure, we found out that there was a fungal infection in some pupae. We went for further fungal culture and diagnostic steps including the histology procedures and Koch’s postulates. As a result, we discovered that Fusarium sp. could penetrate through the pupal cuticle and be responsible for the mortality of some pupae. (Fig. 7)

Figure 7: R. pomonella infected by fusarium sp.
Research conclusions:

This research is the first report about the efficiency of entomopathogenic nematode against the soil-dwelling state of R. pomonella under field conditions. Our study indicated that:

a) High dose of entomopathogenic nematode S. riobrave (200 IJ/cm2), as a biological control agent, could suppress significantly the soil-dowelling stage of R. pomonella under field conditions, especially during the fall seasons. In other words, the application of S. riobrave on the orchards can reduce the survival rate of apple maggot fly pupa in the soil.

b) Our laboratory research showed that the suppression effect was higher in the non-autoclaved soil where EPNs were applied to the soil against plum curculio in July 2020.

c) Combined application of two EPNs, S. carpocapsae and S. riobrave, significantly led to suppression of the R. pomonella pupae under laboratory conditions. 

d) Primarily data revealed that Fusarium. sp had a deteriorating effect on the emergence rate of R. pomonella pupa.


Participation Summary
4 Farmers participating in research

Education & Outreach Activities and Participation Summary

74 Online trainings
1 Published press articles, newsletters
1 Webinars / talks / presentations
1 Workshop field days
1 Other educational activities: 1 Webinar (March 3rd, 2021), Presentation title: Advances in the control of fruit flies; Entomological Society of America.1 Field day (May 20, 2021) to 36 fruit growers at the UMass Cold Spring Orchard Research and Education Center. 1 video presentation with 142 online participation in the Soil Health Innovation Conference (March 2021)

Participation Summary:

4 Farmers
Education/outreach description:

The product of this project included online participation in the Soil Health Innovation Conference (March 2021) with video and poster presentation which leads to achieving second place in the competition section. The prepared video has been observed by at least 150 national growers and researchers. Moreover, one extension document was published on a fruit note article. 

Project Outcomes

41 Farmers reporting change in knowledge, attitudes, skills and/or awareness
Project outcomes:

This study satisfied critical needs regarding control of soil-dwelling stages of apple maggot pupae in apple orchards which in turn can bring promising outcomes for biological pest control via entomopathogenic nematodes, as an ecological friendly agent, to reduce the amount of pesticide and bring more sustainability. For instance, by applying less pesticides there could be improved soil microbial community and less pesticide residue in fruit products.

This study determined that the application of S. riobrave under apple trees where most pupae overwintered could significantly suppress the adults' emergence rate. Moreover, fall evaluation indicated that this biological control agent could be persistent and active on the soil during the fall and winter. Specific knowledge gained in the laboratory included insight to use the optimum dosage of EPNs, predation rate, persistence, and activity of these nematodes as evaluated using soil collected from Cold Spring Orchard after receiving EPN applications in July 2020. This described that S. riobrave and S. carpocapsea can be persistent on the soil under an apple tree canopy, and their survival will not diminish via harsh winter. This field research was done for the first time against a soil-dowelling stage of apple maggot pupa emergence rate and provided a valuable turning point to develop functionally integrated systems.

Knowledge Gained:

Through the execution of this research project, my advisor and I learned about the performance and potential use of entomopathogenic nematodes (EPNs) as biological control agents of a key apple pest. Specific information was not available about whether EPNs were able to (1) attack the pupal stage of the insect, in the soil (we got positive results) and (2) whether EPNs applied in the fall, under colder conditions, would be able to kill the pest (positive results).

In addition, knowledge was gained when via experimentation, I discovered that one species of Fusarium (a fungus) was able to kill apple maggot pupae. This is important because we now understand that various types of organisms (EPNs, fungi) can kill the pest in the soil.

Stakeholders (e.g., apple growers) gained knowledge from the presentations (poster, oral). Those presentations acknowledged the important support provided by Northeastern SARE.

Information Products

Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture or SARE.