Final Report for LS94-057
Take-all was severe in continuous wheat rotations throughout the study. Take-all was just as severe following millet as after soybean. Results from the 1996 and 1997 showed that a one year rotation with canola prior to wheat had a significant effect on suppression of take-all root rot. Wheat grain yield was the same, and in most rotations test weight, and 1,000 kernel weight were the same as the non-diseased controls following canola. This was a greater yield improvement than anticipated because of the severe damage from take-all in continuous wheat during all three years. Yield components were significantly lower in rotations with continuous wheat. The possibility that the rapid decline in take-all after canola may be due to compounds released during decay of canola tissues is being investigated. Assays in a controlled environment chamber for take-all on wheat seedlings grown in soil from field plots were similar to results from field trials. The incidence of infected plants and root rot severity were greatly reduced on seedlings grown in soil from rotations with canola. Data for disease incidence and severity from the field prior to wheat harvest were similar to data from seedling assays. The beneficial value of canola in wheat rotations for take-all control has been incorporated into Extension recommendations in Georgia.
The population of Hessian fly on wheat was below economic thresholds during 1995. During 1996 and 1997, winter infestations were significantly lower when canola was rotated with wheat. By 1997 Hessian fly infestations were greater following millet than after soybean. Spring infestations were not affected by the previous crop. False chinch bugs were present in canola in the first season but could not be assessed in 1996 due to the severe winter freeze which killed canola. Millet stands were reduced by false chinch bugs following canola in 1996 and 1997. True chinch bugs, which often damage millet seedlings after small grains, were not found. Thrips populations were higher on soybean seedlings after canola than after wheat, but thrips are not damaging to soybean. Fall armyworm, southern green stink bug, and leaffooted bug attacked whorls and seed of millet but their populations were not influenced by crop rotation. Insect pest populations on soybeans were not affected by the preceding winter crop. Only soybean loopers and velvetbean caterpillars were above economic thresholds. More lepodopterans were found on soybeans following rye. The primary effect of rotation sequence was on seedling insect pests.
The stand of soybeans was reduced 20% or more following canola each year which could not associated with insect damage. Pearl millet stands were also significantly lower after canola but not wheat. This is the first evidence that canola may have an allelopathic effect from breakdown products in canola stubble on these crops in a doublecrop rotation.
Rotation effects were significant for pearl millet panicle counts, leaf blight, and stalk and neck rot during the 1996 season. There was a trend for leaf blight and stalk and neck rot to be less severe in pearl millet following canola than following wheat. Seedling stand was positively correlated with leaf blight and stalk rot. Rotation effects on pearl millet diseases may have been obscured by variation in stand density within subplots. Minor effects on pearl millet stand establishment, retention of green foliage, stalk and neck rot, smut, and yield were observed in these rotations. Seedling stands 3 weeks after planting were lower following canola than wheat in 1995 and 1997. Foliar chlorosis and necrosis were slightly less severe in pearl millet following canola than wheat in 1995 and 1996. Stalk and neck rot was also less severe following canola in 1996. Grain yield was highest following canola in 1997 but may be an artifact of excessive stand density. A trend toward increased smut severity in plots continuously planted to pearl millet was observed in 1997.
Canola diseases were not significant during 1995 and could not be assessed because the crop was killed by low winter temperatures in 1996. After two seasons, there were no differences in severity of stem canker on soybeans due to crop rotation or the inclusion of canola or pearl millet in the rotation. Sclerotia stem rot and black leg did not become serious during the three-year period, probably as a result of loss of the crop in 1996. The rotations will be continued in 1998 to assess rotation effects on these diseases.
Soybean yield was reduced following canola probably because of allelopathic effects from the decaying canola stems and roots. Various foliar diseases were present but did not cause economic loss in any year. Stem canker increased in severity early in the 1997 season in continuous soybean rotations but did not affect seed yields. Additional data will be collected in 1998 on rotation effects on stem canker. Canola and pearl millet were compatible with soybean in the rotation. No changes in disease management is foreseen to incorporate these crops into rotations with soybean.
Analyses of the income returns from the rotations was done by developing cost and return budgets for each crop by year using plot yields and field operation and input costs realized by the Southwest Georgia Branch Experiment Station. The analysis shows that crop failure and crop prices influence rotation effects on returns. These influences can magnify negative relationships such as reduced yields of soybeans behind canola, and/or shrink positive benefits such as take-all reduction on wheat from having canola in the rotation. High soybean prices and wheat prices may make the reduction in soybean returns following canola less than the increase in wheat returns from having canola in the rotation ahead of wheat. In contrast, beneficial but costly winter cover crops with no income (rye) and/or low income potential neutral crops such as millet can make a rotation unprofitable or add no positive economic benefits. However, the low income of millet could be an improvement over soybeans after canola. The results indicate the current vulnerability of the new crops canola and pearl millet. Improvements in varieties adapted to the region and improved yield potential will make these crops more profitable.
Video footage showing the results of the rotations on diseases and insect pests to date in field plots have been made. Information on the procedures used and the biology and damage caused by the various pests have been documented. Aerial footage showing the plots during May to document the effect of rotation on take-all of wheat has been recorded. Film will be edited to complete the educational video based on the study. Several training programs and presentations at various meetings have been completed and additional presentations will be made in 1998. Audiences varied from small acreage to large-scale growers, extension specialists, and researchers throughout the Southeast. Several technical and nontechnical publications have been completed. Refereed journal publications are being prepared from the research on rotation effects on take-all, insect populations, and pearl millet diseases and crop yields. A comprehensive publication on the project will be prepared as a College of Agriculture Research Bulletin.
Disease and insect pests became serious problems in the Southeast when wheat and soybean were grown in continuous double cropping and minimum tillage systems. Therefore, the objectives of the project are to:
1. Enhance double cropping systems with minimum tillage in the southeastern U.S. by expanding crop rotations which can be profitable and which can reduce diseases and insects.
2. Incorporate improved cultivars of the emerging crops canola and grain pearl millet into minimum tillage systems.
3. Determine the optimal rotation system to manage diseases and insects in canola, pearl millet, soybeans, and wheat.
4. Demonstrate the usefulness of these rotations to growers on a commercial farm and at a major regional farm exposition site.
Wheat acreage increased from <122,000 ha per year in Georgia in the early 1970s to 607,000 ha in 1981. Wheat was often planted four or more years without rotation. As a consequence, take-all root and crown rot, caused by the fungus Gaeumannomyces graminis var. tritici (Ggt), became a significant limiting factor by the early 1980s. A natural decrease in disease severity called `take-all decline' occurs where wheat is grown as a single crop each season in monoculture due to the buildup of antagonistic microflora in the soil over several years. Take-all decline did not to occur in the Southeast with continuous double cropping, probably because of a disruption in the population of antagonists as a result of growing soybean between wheat crops. Rye and barley planted into a take-all infested site as rotational crops had little damage from take-all, but sufficient inoculum was maintained so that wheat planted the following year sustained as much yield reduction as following continuous wheat. Oats is the only winter cereal crop that can be rotated with wheat to suppress the take-all fungus. A noncereal crop such as canola may provide a useful alternate rotational crop between wheat crops. Continuous wheat in reduced tillage systems also has contributed to devastating outbreaks of the Hessian fly in the Southeast. The Hessian fly is active from the fall to the spring and is the most important wheat pest in the Southeast. The insect aestivates over the summer in wheat stubble, consequently stubble destruction by plowing or disk harrowing can greatly reduce over-summering populations and infestations in the next wheat crop. Crop rotation has long been recognized as beneficial in controlling the Hessian fly, but this benefit has not been measured for any wheat pest in double cropping systems used in the Southeast. Cropping sequence can influence arthropod pest populations. A pest may increase in a crop and be present when the subsequent crop is planted. This problem is magnified in doublecrop systems because crops are planted soon after harvest of the preceding crop. No-tillage may enhance pest survival between crops by providing a more suitable habitat than conventional tillage. Chinch bug, Blissus leucopterus leucopterus (Say), builds up in winter small grains before moving to crops such as corn and sorghum. Chinch bug is an important pest of pearl millet in Georgia (R. Hudson, personal communication). Planting pearl millet after small grains may enhance chinch bug infestations. False chinch bug, Nysiis raphanus Howard, also damages soybean seedlings when soybean is no-till planted into canola stubble. Canola may also be an early season host of many polyphagous insects including aphids, thrips, and stink bugs that are pests in summer crops. Southern stem canker of soybean increases in severity in the no-till double cropping system. Incidence and severity of stem canker on susceptible cultivars have been at least twice as high in no-tillage plots as in plots with conventional tillage. Increases in disease incidence have been dramatic when the no-tillage, doublecrop system is sustained. In three years of sustained no-tillage double cropping with weather favorable for disease, the percentage of dead plants in no-tillage plots increased from 2 to 40 to 60. The percentage of dead plants in comparable conventional tillage plots increased from 1 to 11 to 15. These disease increases in no-till culture are probably a direct result of increased invasion early in the season which results from abundant inoculum arising from undecomposed stems from the previous soybean crop. A consistent modest increase in disease severity in the soybean/wheat system compared to soybean/fallow has also been observed. This is probably an indirect result of microclimate differences caused by the presence of wheat stubble. A pearl millet hybrid has been released for use as a summer grain crop. The grain makes a high-quality poultry feed. Because of the importance of poultry to the Southeast, a large potential market exists. The growing season for pearl millet is flexible enough for planting after wheat or canola or harvesting early enough to precede the recommended planting dates for fall planting of these crops. Data on disease and insect pests of pearl millet in doublecrop rotations in the Southeast are limited.
Twelve rotation sequences instead of ten described in the proposal were included in the study. Rotation sequences for are presented for three years. The first crop was planted in the fall and the second crop in each set was the summer-planted crop. Each sequence was planted at the Southwest Branch Station (Plains) as a randomized complete block with four replications (each plot 40 X 40 ft), and as a single replicate (each plot 20 X 200 ft) at the Speir farm (Plains) and at Sunbelt Expo (Moultrie).
Take-all on wheat and rye. The take-all fungus Gaeumannomyces graminis var. tritici was grown on autoclaved oats and the cultures were air-dried. This inoculum was ground with a hammermill to pass through a 7 mm mesh within one week before application to the field plots. The dry inoculum was spread on the surface of each plot at the rate of 3.6 g/m2 at the Southwest Branch Station at Plains, GA. A 6X40 ft area on the far right side of each plot was not inoculated to serve as a control. The inoculum was incorporated into the soil to a depth of 15 cm just prior to planting.
Savannah wheat and Wrens Abruzzi rye were planted November 9, 1994 and November 10, 1995. Savannah wheat was planted November 12, 1996. Seed was planted in a 7-inch row width with a Hege small-plot grain drill at 2 bu/acre. Rye was removed from plots on March 10, 1995 and March 3, 1996 to simulate winter grazing and its use as a winter cover crop. Plots were killed with a herbicide.
Wheat and rye plants were dug for evaluation of take-all damage when plants were at the seed milky ripe stage. Plants were removed from randomly selected sites determined by specifying a distance from the end of each of five sections of a plot using a random number table. Thirty-five plants of each crop were collected from seven noninfested sites in each plot. Soil was washed from roots and each plant was rated visually for root and crown rot using the 0-4 scale (Shipton, 1972). Data were collected from a minimum of 146 plants per rotation sequence from sites with Ggt-infested soil. The percentage of diseased plants and disease severity rating was assessed.
The number of tillers per meter of row was counted when seed was near dough stage of maturity (early May) about two weeks before wheat harvest. Five random row lengths in each plot were sampled using the same procedure used for collecting root samples. Counts were made in Ggt-infested and non-infested areas. These subsamples were used to determine the average number of tillers per replication of each rotation. Grain from a 1,000 square foot area in each plot was harvested with a Hege plot combine. Grain yield, test weight, and 1,000 kernel weight were determined for each plot. Grain weight was adjusted to 13% moisture.
Soil was collected during the spring when wheat was at the heading stage and when Ggt was actively parasitizing the roots and basal stems. A second sample was collected each year in late summer when the summer crops were approaching maturity. Soil and associated plant roots in the upper 20 cm were collected randomly within the portion of each plot infested with Ggt, the noninfested portion of each plot, and from a nearby area outside the plots. Soil from each plot was dried and homogenized by grinding. In the first two assays (1995), field soil was placed into pots at two rates: 100% field soil or soil diluted 10-fold with soil collected in the same field but from a site not infested with Ggt (10% field soil). This was done to have at least one assay that would exhibit a quantitative differential between treatments. Disease severity was greater in the 100% soil, and the relative differential between treatments was the same. In subsequent assays only 100% field soil was used. Soil samples were collected March 15 and August 30, 1995, April 18 and September 19, 1996, and April 1 and September 16, 1997. Data from the September 1997 assay are not yet available. Summary data are presented for the first five assays (Table 3). Data in all tests were analyzed by analysis of variance and means separation tests.
Insects. 'Iris' canola was planted in assigned plots on November 8, 1994, November 7, 1995, and November 11, 1996. Canola was planted in 7-inch row with a Hege small-plot grain drill at 6 lb/acre. Canola stands were killed in 1995 by cold temperatures in December and were replanted on March 5, 1996. Canola grain yield was measured in plots on May 23, 1995 and May 15, 1997 by harvesting a 5 x 40 ft area of each plot with a Hege small-plot combine. Seed was weighed, and moisture content and test weight measured. Grain yields were adjusted to 8.5% moisture. Canola grain yield was not measured in 1996 because plants from the second planting were beginning to flower in late May. Plants were killed with gramoxone and mowed with a rotary mower before planting summer crops.
Hessian fly infestations were sampled in wheat plots February 1 and May 9 in 1995, January 26 and April 18 in 1996, and February 5 and April 24 in 1997 by collecting plants in 2 subsamples of 1-ft of row per plot. Tillers were dissected and the number of larvae plus puparia were counted; the percentage of infested tillers (stems) was determined.
Soybean and grain millet plant numbers were counted at 20, 21, and 20 days after planting in 1995, 1996 and 1997, respectively, by counting plants in two 10-ft sections of row in each plot. Numbers of false chinch bug, Nysius raphanus Howard, were determined in each plot from three 1-ft2 areas of ground centered over a row of plants. Soybean seedlings were examined for thrips by collecting 20 plants per plot in 1995. Plants were placed in berlesse funnels for 72 hr to extract thrips and counted. Foliage-inhabiting insects were sampled in soybean using the shake-cloth method (Kogan & Pitre 1980). Beginning in early July of each year and every two weeks until harvest, two 60-cm of row shake-cloth sampled were collected per plot. The method consists of placing a white, plastic cloth between two rows and vigorously shaking plants in two 30-cm sections of row over the cloth for 15 sec. All important phytophagous and predatory arthropods are counted.
Grain millet was sampled for whorl damage by the fall armyworm, Spodoptera frugiperda (J.E. Smith), on August 8, 1995 and September 5, 1996 by counting plants with whorl defoliation in 10-ft of row per plot. Insects feeding on seed were sampled on August 31 and September 20, 1995 and September 5 and 19, 1996 by inspecting 10 seed heads per plot and counting all insects observed. In 1997, 20 seed heads were sampled per plot September 4 and 17 and October 1.
Plant stand and insect counts were averaged among plots with similar winter crops. Means were analyzed by sample date with an analysis of variance for a randomized complete block design. Means were separated with a protected least significant difference test (LSD). Single degree of freedom contrasts also were used to compare the effect of previous crop combinations.
Pearl millet diseases. Soil borne pathogens of pearl millet Sclerotium rolfsii, Bipolaris setariae, and Exserohilum rostrata were cultured individually in flasks containing sterile oat grains for 4 weeks. After removing from flasks and drying, approximately equal quantities of inoculum of each of the pathogens were mixed, and inoculum (250 grams, approximately 1 liter) was distributed throughout each plot area on June 7,1995.
Pearl millet HGM 100 was planted on June 8,1995, June 19,1996 at 2.8 kg seed/ha. A calibration error resulted in planting rate on 24 June 97 of approximately 13.5 kg seed/ha. Rows were spaced 0.9 m apart. Four 3 m long, two row subsample areas were marked within each plot. Stand counts within subsamples were made on July 5,1995, July 10, 1996, and July 15, 1997. Final panicle counts were made on September 19, 1995, September 3, 1996, and September 23, 1997.
Disease assessments were made within subsample areas. Visual estimates of leaf blight severity (percent leaf area chlorotic or necrotic) were made on September 19, 1995, and August 26, 1996. Culms with stalk and neck rot and total culms were counted September 20, 1996. Smut severities (% infected florets within panicles) were estimated on September 23, 1997. Four rows (12.2 m long) of the main plots were harvested on September 21, 1995, September 20, 1996, and September 23, 1997 with a Hege combine. Harvested grain was dried in a forced air oven for four days at 38 C prior to determining yield. Twenty panicles were harvested from each subsample area in 1996, threshed, and average panicle yield was calculated. Subsample yield was calculated as the product of panicle number and average panicle yield.
Data were analyzed within each year. Mean squares for analysis of stand counts, panicle counts, leaf blight, percentage stalk and neck rot, and smut severity were partitioned into effects due to replication, rotation, and subsample. Mean squares for analysis of plot yield were partitioned into replication and rotation effects. Correlations between stand and panicle counts, panicle yield, subsample yield, leaf blight, and percentage stalk and neck rot were calculated.
Canola diseases. Canola was monitored during the rosette, the flowering, and the pod filling stages for symptoms of Sclerotinia stem rot.
Soybean diseases. Soybeans, varieties Brim and Ga 81-2057, were planted on June 15, 1995. The variety Deltapine 105 was planted on June 19, 1996. The soybeans were planted into standing residue of the previous crop. Soybeans were monitored for symptoms of stem canker, from flowering until harvest. Final stem canker ratings were made near physiological maturity each year. The plots of both crops were also periodically observed for the presence of any unexpected diseases. Soybean plots were harvested on October 20, 1995 and on October 16, 1996. A 200 square foot area of each plot of each variety was harvested in 1995 and a 400 square foot area was harvested in 1996. Reported yields were adjusted to 13% moisture content.
Take-all. Take-all was severe during the first year with wheat yield reduced >75% below that of the healthy controls. The percentage of infected wheat plants in 1996 was reduced significantly in rotations where canola was planted in 1994. The average root disease severity score was about one-half as much as with canola planted the previous year as continuous wheat. Disease severity was less in the continuous wheat rotation in 1996 with pearl millet as the summer crop in place of soybean, but he difference was not statistically significant. Incidence of disease (percent infected plants) was not affected with nearly 100% of plants infected for both rotations (Table 1).
The incidence of infected rye plants was one-half that of wheat and disease severity was much reduced in 1996 (Table 1). After two years of rye (rotation 12), the incidence and severity of take-all in wheat in 1997 did not differ from continuous wheat (Table 2). This agrees with previous results for the susceptibility of rye. However, wheat grain yield was significantly higher following rye grown as a winter cover crop than continuous wheat. Previous research (Rothrock and Cunfer, 1991) showed that wheat following a rye grain crop had as much damage as continuous wheat. Additional data from other rotations were not available in this study. Therefore, these results need further validation. Take-all incidence and severity on wheat and rye plants increased each year in the noninfested control area, but were similar to rotations with canola the previous year. Rye, whether grown as a grain or a cover crop in fields with severe take-all, is less reliable to reduce take-all than other non-host crops.
Take-all caused severe yield reduction in the continuous wheat rotation sequences (rotations 1 and 9) in 1996 and 1997 (Tables 1 and 2). Grain yield in rotations in which canola was grown as the winter crop in 1995 did not differ from the controls, ie wheat harvested from portions of the test site not infested with Ggt. Similar results were found for grain test weight and 1,000 kernel weight (Table 2). These results show that canola can be planted as a cash crop for one season in sites heavily-infested with the take-all fungus and reduce inoculum levels below an economic threshold. Currently oats is the only fall-planted small grain that reduces take-all damage in a following wheat crop. Oats is a much less profitable crop than canola is expected to be. Therefore, canola can diversify crop choices for a grower with a potential increase in profitability and serve to control take-all in wheat.
Soybean maintains Ggt inoculum at a high level. Comparisons are made in this study to compare pearl millet to soybean as the summer crop for their influence on survival of Ggt and yield components in continuous wheat production. In 1995 no differences in yield components were found when wheat followed pearl millet or soybeans. In 1996 grain yield was not higher following millet but test weight and 1,000 kernel weight were significantly higher compared with soybeans as the summer crop (Table 2). However, in 1997 there were no differences in wheat yield or disease parameters between the two continuous wheat treatments with either continuous soybean (rotation 1) or continuous millet (rotation 9) as the summer crop (Table 2). Millet in the rotation does not reduce take-all.
Data from the field on take-all were corroborated with assays for development of take-all on wheat seedlings grown in soil collected from field plots on five sampling dates (Table 3). Data from the fourth sample date are lower than expected, possibly because seedlings were removed from growth chambers before symptoms were at the maximum. Results from April, 1997 (date 5) are more comparable to earlier results. Where no wheat or rye has been grown during the first two years (rotations 2, 4, and 6), take-all severity declined to less than 0.7 compared with 3.2 with continuous wheat (0-4 scale with 4 being most severe) in 1996. Disease severity for wheat in 1996 following canola in 1995 (rotations 3, 8, and 11) declined to 1.9 or less. Disease severity also declined in the seedling assay to 1.1 or less in rotations with wheat in 1995 and canola in 1996 (rotations 5 and 7). Incidence and severity has stabilized in continuous wheat (rotation 1 and 9) with incidence remaining >90%. Similar to field results, millet or soybean did not differ in their effect on Ggt survival and take-all severity. The incidence of take-all is in general agreement with the disease severity results. In the rotations with no wheat, incidence of seedling infection decreased to 31-60% by the third sampling date compared with 100% in continuous wheat. In rotations with wheat followed by canola, incidence was <80%. When wheat followed canola, incidence at the third sampling was 85% or less. By 1997, rotations with canola reduced take-all incidence to less than half that for continuous wheat in most cases (Table 3). Insects - Winter Crops Wheat. Because the study began in fall 1994, no rotational effects were established in the 1994-95 crop season. Hessian flies were not recovered from wheat in the February samples but averaged (+SD) 14.2 + 7.7% infested tillers and 0.39 + 0.07 immature per tiller in the spring samples. In 1995-96, winter infestations were significantly greater where wheat was the previous winter crop compared with wheat where canola was the previous winter crop (Table 4). Spring infestations were not significantly affected by previous crop. In 1996-97, both winter and spring infestations generally were greater in wheat following wheat than following canola (Table 5). This contrast was significant only for the spring infestations. Previous summer crop did not affect Hessian fly infestations in 1995-96, but infestations were greater following millet and than following soybean in 1996-97. Canola. Canola grain yield averaged 1619 + 49 kg/ha in 1995 and 1516 + 98 kg/ha in 1997 and was not significantly different between plots in either year (Table 6). Because a severe freeze in December 1995 killed canola in all plots, no grain was produced in the 1995-96 season. Plots were replanted in March 1996 and plants were bolting in mid-May when they were killed to prepare for planting summer crops. False chinch bugs were present in canola plots at harvest in 1995, but population numbers were not determined. No insect results were collected in 1995-96. In 1997, one generation of tarnished plant bug, Lygus lineolaris, developed on plants between flowering and harvest (Table 7). Large number of false chinch bugs also were present immediately before harvest (Table 7). Insects - Summer Crops Grain millet. Millet plant stands were not significantly different between cover crops in all years, although plant numbers were lower following canola than wheat or rye in 1995 (Table 8). False chinch bug were present in most millet plots following canola in 1995 with infestations in 2 of 8 plots exceeded 10 bugs/ft2 (Table 8). Millet seedlings in these plots were stunted and killed by bug feeding resulting in extensive gaps in the stand. Chinch bug numbers were much lower on millet in 1996 and 1997 than in 1995. Millet was attacked by the fall armyworm during the whorl stage, but infestations were not affected by previous winter crop in any year (Table 9). The two seed feeding insects were the southern green stink bug, Nezara virdula (L.), and leaffooted bug, Leptoglossus phyllopus (L.). Both insects sucked seed contents causing seed to be shriveled and deformed. The southern green stink bug numbers generally were not affected by previous crop in any year except on the second sample data in 1995 when stink bugs were more prevalent in millet following canola than wheat or rye (Table 9). Leaffooted bugs also were not different between winter crop treatments in any year. In 1997, large numbers of the corn earworm, Helicoverpa zea, were feeding on millet seed heads on September 4, but a viral epizootic virtually eliminated earworms by the September 17 sample. Large numbers of southern green stink bugs also caused considerable injury to seed on millet seed heads in 1997. Soybean. Soybean plant stands were significantly lower following canola than wheat or rye in all years (Table 10). False chinch bugs were somewhat more abundant following canola than wheat in all years, but little visible injury to seedlings was observed in any year. Thrips also were more abundant on soybean following canola than wheat in 1995 (Table 10). Predominant defoliating insects in soybean were the green cloverworm, Plathypena scabra (F.), soybean looper, Pseudoplusia includens (Walker), and velvetbean caterpillar, Anticarsia gemmatalis Hubner. Other phytophagous insects included the bean leaf beetle, Ceratoma trifurcata (Forster), spotted cucumber beetles, Diabrotica undecimpunctata howardi Barber, threecornered alfalfa hopper, Spissistilus festinus (Say), and stink bugs (>95% were N. virdula, others were brown stink bugs, Euschistus spp.). Predominant predatory arthropods were big-eyed bugs, Geocoris sp., damsel bugs, Nabis spp., and various spiders. Populations of soybean loopers and velvetbean caterpillars were above economic threshold and produced defoliation levels exceeding 50% in all plots by late September in 1995 and 1997.
Winter crops did not significantly affect populations of any phytophagous taxa any sample date in 1995 (data not presented). However, when averaged across the entire season in 1995, more soybean loopers and consequently total lepidopterans occurred on soybean following rye than canola (Table11). Numbers on soybean following wheat were not different from numbers on soybean following canola or rye. Likewise, soybean predator populations also were not significantly affected by winter crop on any sample date, except damsel bugs were more abundant following canola and wheat than rye on the first date that damsel bugs were collected in 1995. In 1996, winter crop did not significantly affect the seasonal average number of any arthropod taxa except that southern green stink bugs were were less abundant following rye and canola than following wheat (Table 12). However, stink bug number were very low in all plots in 1996. In 1997, winter cover crop also did not significantly affect seasonal average number of any arthropod taxa (Table 13). Over all years, winter crop did not consistently affect any foliage-inhabiting arthropod taxa on soybean.
Pearl millet diseases - 1995. Rotation had a significant effect on stand counts (P<0.0001). Pearl millet following canola tended to have lower stand counts than that following wheat or rye (Table 14). Rotation had a small effect on panicle counts (P=0.09), which were highest following rye. Yield was affected by rotation (P=0.05) and was lowest in rotation 4, which also had the lowest stand counts. Pearl millet yield following canola did not consistently yield less than when following wheat, as evidenced by rotation 8. Rotation had a small effect (P=0.10) on leaf blight. Pearl millet following canola tended to have less chlorosis and necrosis than that following wheat. 1996. Rotation effects were significant for panicle counts, leaf blight, and stalk and neck rot (Table 15), although there were few consistent effects due to the preceding crop. There was a trend for leaf blight (symptoms were consistent with those caused primarily by Exserohilum rostrata) and stalk and neck rot (caused primarily by Fusarium roseum and Bipolaris setariae, respectively) to be less severe in pearl millet following canola (rotations 2 and 7) than following wheat (rotations 3, 8, 9, and 11). The effects may be obscured by the high stand density of rotation 6, which resulted in relatively higher values for leaf blight and stalk and neck rot. Correlation coefficients among yield components and disease data indicated that stand density had a significant effect on yield components and disease within subsample areas (Table 16). Seedling stand was positively correlated with leaf blight and stalk rot. The higher stands within subplots of rotation 6 probably resulted in greater severity of leaf blight and stalk rot compared to disease in rotations 2 and 7 (Table 15). The 1996 rotations indicate that the previous crop had no consistent effect on pearl millet grain yield, however, high seedling density promoted leaf blight and stalk rot. 1997. A calibration error in planting the pearl millet plots resulted in excessively high stand density. Problems associated with the high stand density included extreme variation in plant development within the rows. Panicles ranged in size from about 8 cm to over 30 cm long, and many plants failed to form panicles. With the intense competition for resources, plants matured rapidly after pollination and before differences in leaf blight and stalk and neck rot could be evaluated. In spite of the excessive stand, seedling stand was lowest in millet following canola (Table 17). The effects of canola may be somewhat long-lived, since even plots planted two years after canola (rotations 4 and 5) tended to have lower stands than those in which canola had not been planted at all (rotations 9 and 12). The high stands in rotations 9 and 12 resulted in greater panicle numbers. Seedling stand and panicle number within subsample areas were significantly correlated (R=0.51, P<0.0001). Smut was significantly greater in plots that had been planted continuously to pearl millet over the three years (rotation 9). Smut severities were lowest in plots that had not been previously planted to pearl millet (rotation 5), but the differences with the other treatments were not significant. Grain yield was greatest in plots following canola (rotation 3). Higher yields following canola have not been observed in previous years, therefore it is likely that the greater yield was the result of a slight advantage in growing conditions resulting from the lower stand density. Canola diseases. No symptoms of Sclerotinia stem rot were observed on canola during 1994-1995. This was probably a result of dry weather conditions during the flowering period. A period of several days of saturated soil is required for germination of sclerotia and production of ascospores. To complete the infection cycle, this period must be followed by a period where the foliage and flower petals remain wet. This apparently did not happen during the critical flowering period in 1995. The crop was frozen in the seedling stage in December 1995 and could not be replanted until March 1996. Thus, there was no canola present during the usual infection period and by the time the replanted crop was flowering it was too hot for infection. As a result, no infected plants were found during 1996. Very light and scattered infections of white leaf spot, downy mildew, and Alternaria pod infections were observed each year but were not severe enough to be rated. The anticipated disease problems of canola have not developed at this stage of the study. This may be due, in part, to the loss of the second year canola crop. In 1996-97 the disease situation was similar to previous years. Light and very scattered development of white leaf spot and downy mildew was observed on leaves and stems, and Alternaria was present on a low percentage of pods prior to maturity. The major diseases, blackleg and Sclerotinia stem rot, were not observed at any stage of crop development. Both have been observed at the Plains Station previously and stem rot was observed at other locations on the station in 1997. The absence of these diseases from these plots in 1997 was unexpected and is difficult to explain. Two factors probably contributed to this situation, a relatively thin stand and the loss of the 1995-96 crop to freezing. The canola stand was adequate for good yield but was thinner each year than the stand usually obtained in commercial fields. The thin stand density and relatively wide plot borders may have allowed sufficient air circulation within plots to promote rapid drying of plant surfaces and thereby reduced the potential for pathogen invasions. The 1995-96 crop was not reestablished until mid-March. This three month period is the normal invasion time for both blackleg and stem rot. The absence of the crop during this period may have been as effective as a one year rotation in interrupting the development of these diseases. Soybean diseases. Stem canker developed to moderately severe levels in both 1995 and 1996 (Table 18). The low yield of Ga 81-2057 is probably primarily a result of damage from stem canker. In the absence of disease Ga 81-2057 and Brim should have produced similar seed yields. Both Brim and DP 105 are considered to be susceptible to stem canker, whereas Ga 81-2057 is extremely susceptible. The stem canker ratings of Brim and DP 105 indicate that disease severity was higher in 1996 than in 1995. There was no significant difference in stem canker severity in either year that could be attributed to previous crop sequence. The low yield of soybeans following canola in 1996 cannot be attributed to differences in severity of stem canker. Although the soybean stand following canola was significantly reduced in 1996, this was also true in 1995 when the yields were not different. In 1996 a comparison was also possible between soybeans following two successive winter crops of canola and those following only one. That comparison indicated no significant difference in stem canker severity or in yield. One possible explanation involves the replanted canola that had to be mowed at the early flowering to permit planting of the summer crops in 1996. This immature canola could have been much higher in glucosinolates than normal mature canola and could have released much higher levels of phytotoxic decomposition products. There is no evidence that inclusion of pearl millet as a summer crop and canola as a winter crop has increased the severity of any disease of soybean. Conversely, there is also no evidence that inclusion of these crops into the double-cropping system has reduced any of the soybean diseases. Changes in disease intensity may become evident after additional cycles of the rotation are completed. In 1997, brown spot, downy mildew, and anthracnose were present throughout the season but were very light and scattered. A few plants had symptoms of red crown rot and a few were killed early in the season by an unidentified cause. None of the diseases reached a severity that would be expected to influence seed yield. Stem canker incidence and severity were higher than previous years. The incidence of visible symptoms just before maturity was 38% and many plants died prematurely. This high level of stem canker contributed to the low seed yields and the extremely dry weather during pod fill also severely depressed yields. The mean yield of 12.3 bu/acre was the lowest recorded during this study. For the first time in three years, early season observations indicated that there was a difference in stem canker incidence due to previous crops. There appeared to be a reduction following canola and an increase following millet. In the final disease counts, these differences were not significant (P=0.05) and were not reflected in seed yield. Additional rotation cycles will be needed to determine if there is a developing influence of previous crops on stem canker incidence. Problems encountered. All major research objectives will be met. However, as anticipated at the start of the project, more data than can be determined in three years is needed. The major problems involved the demonstration plots at SunBelt Expo and the Speir farm at Plains. The plots at both sites required more on site supervision than anticipated. Shortly after the project began, strong disagreements between the management of SunBelt and College of Agriculture administration resulted in reduced activity by the University of Georgia at the Expo. Also less time available by the Extension PIs because of increased workload due to continuing reductions in state funding to the College made timely management of this site difficult. In addition, one of the Extension PIs, Boyd Padgett, left the University for another position in 1996 and his position was not refilled because of budget constraints. The Speir farm site was useful but more work needed to be performed by the Branch Station staff than anticipated. Mr. Speir attended most project meetings but could not be persuaded to try canola on his farm although a number of farmers in nearby counties have experimented with the crop on their farms. This was probably due to the fact that peanut and particularly cotton prices have been very good in the past three years and he committed most of his acreage to those crops. On farm demonstrations have educational value but obtaining reliable research data is more difficult. The only problem at the research site was the freezes during the 1995-96 season resulting in loss of the canola crop. This resulted is less disease buildup than expected and reduced the amount of data collected during the project. More data should be available by the spring of 1998. The loss of the crop showed the need for better adapted cultivars to make this an economical crop in the region. Role of Southern SARE in meeting objectives. Southern SARE has provided satisfactory assistance with administration of the budget and has been responsive to some minor adjustments that were needed including no-cost extensions. Multidisciplinary research on entire cropping systems is often difficult to facilitate. The project allowed us establish large field plots for an extended period. Small plots are much less conducive for study of plant pests which move easily from site to site. It also gave the opportunity to follow specific rotation sequences which normally are not possible. Partial funding to continue the project has been obtained and other funds are being sought. The project has been modified for the 1997-98 season to emphasize more rotations with canola in the winter and cotton will be added in the summer and millet reduced. The recent increase in cotton in the Southeast, often with continuous production, is raising the same concerns about continuous wheat:soybean this project addressed. Take-all will continue to be monitored as will Sclerotinia rot of canola and stem canker of soybeans and other diseases and both harmful and beneficial insects on all crops. Although this project focused on pest management, it also shed light on other aspects of crop management. There have been anecdotal reports of reduced plant stands in several crops following canola, including cotton. We found sound evidence of this in millet and soybean. We will investigate the effect of canola on cotton stands next year. The project has given a multi disciplinary team the support to initiate a long-term study of field crop rotation systems in the Southeast that can be expanded and bring other researchers into the project as it continues.
Educational & Outreach Activities
Cunfer, B.M. 1997. Effect of crop rotation on take-all root rot of wheat. Proceedings of the Southern Small Grain Workers Conference. Gulf Shores, AL. Pp. 5-7.
Cunfer, B.M. 1997. Pest management in sustainable row-crop systems for the Southeast. P. 124 in: Proc. Ag Showcase '97. Univ. of Georgia College of Agricultural and Environmental Sciences Spec. Bull. 88.
Cunfer, B.M. 1997. Effect of twelve crop rotation sequences on take-all of wheat. (Abstr.) Phytopathology 87:S21.
Cunfer, B.M. 1997. Management of take-all root rot of wheat in the Southeast. 2 p. UGA Extension Plant Pathology. Outbreaks and Updates letter sent to county agents. Web site address: http://www.ces.uga.edu/Agriculture/plantpath/epphomep.html.
Cunfer, B.M. 1998. Effect of crop rotation on take-all of wheat in double-cropping systems. Plant Dis. 81(in preparation).
Cunfer, B.M., Buntin, G.D., Wilson, J.P., Allison, J.R., and Phillips, D.V. 1998. Disease and insect management using new crop rotations for sustainable production of row crops in the southeastern United States. CAES Research Bulletin (in preparation).
Wilson, J.P., Cunfer, B.M., and Phillips, D.V. 1998. Crop rotation effects on yield and diseases of pearl millet. Journal of Production Agriculture (In preparation)
The project was discussed and the field site visited during the Small Grains and Forages Field Day at the Plains Station, in April 1995. Demonstration plots were on display at Sunbelt Expo for visitors during October 1995 and 1996. During July, 1996 David Buntin and Barry Cunfer discussed the project and showed the field plots at Plains to a group of 50 farm managers from the People's Republic of China during a tour of farms and applied agricultural research programs in Georgia.
As more data were generated, educational activities were expanded in 1997. These included posters and oral presentations at the Georgia Wheat-Soybean Expo (Macon, January 28), the Southern Canola Information Exchange (SCIE) tour at Plains (March 21), the Southern Small Grain Workers Conference (Gulf Shores, AL, April 27-30), the UGA College of Agriculture and Environmental Science Ag Showcase (Tifton, August 28), the Carolina Farm Stewardship Association Sustainable Agriculture Conference (Flat Rock - Hendersonville, NC, Nov 14-16), and various county agent training meetings throughout Georgia. A technical poster on take-all was presented by Barry Cunfer at the annual meeting of the American Phytopathological Society (APS) (Rochester, NY, August 9-13). A disease management guide which incorporates conclusions from this project was distributed to county agents in Georgia. This publication is also available on the University of Georgia Extension Service World Wide Web site. Citations to publications completed are listed in section F and are attached to this report.
The venues where information was presented provided opportunities to reach various audiences. The Wheat-Soybean Expo was attended by 40 farmers using conventional practices on large acreages whereas the Carolina Sustainable Agriculture was attended by over 300 part-time and small acreage farmers using both conventional and organic farming practices. Twenty-five research and extension specialists from the Southeast participated in the SCIE tour. The Ag Showcase had about 500 participants including farmers, students, university research and extension personnel, legislators, and the general public. About 80 and 1500 research and extension scientists attended the Small Grain Workers conference and the APS meeting, respectively. The project results will be presented at the 1998 Wheat-Soybean Expo and county agent training meetings.
Buntin, G.D. Effect of crop patterns on insect populations and damage in canola, wheat, soybean, and grain millet rotations. Southern Canola Information Exchange tour, Plains, GA. March 21, 1997.
Cunfer, B.M. Effect of drop rotation on take-all root rot of wheat (poster). Georgia Wheat-Soybean Expo. Macon, GA. January 28, 1997.
Take-all. The results show important implications for the incorporation of canola and pearl millet into double cropping rotations in the Southeast with wheat and soybean. A one year rotation with canola reduces take-all root rot in fields where the disease is severe. Grain yield was the same following one year of canola as in control plots with no take-all. However, wheat roots are still partially diseased and will provide inoculum of Ggt if a second consecutive wheat crop is planted. Continuous wheat had severe take-all which resulted in yield reductions up to 75% below the controls. This will allow growers to plant a crop with the potential to increase profitability significantly above planting oats or a fallow rotation which are the only winter crop alternatives now available to reduce take-all in rotation with wheat. Pearl millet fit well into the rotation with wheat, but take-all was just as severe following millet as following soybean. Rye as a cover crop had variable effects on take-all in a following wheat crop and needs further investigation.
Insects. Hessian fly infestations generally were enhanced by continuous wheat whereas rotation with winter canola reduced infestations in the subsequent wheat crop. Previous summer host also may influence Hessian fly infestations with wheat following millet having greater infestations than wheat following soybean.
False chinch bug damage to seedlings of the summer crops grain millet and possibly soybean may be a problem following canola. This was somewhat unexpected, because it has long been known that true chinch bugs, Blissus leucopterus leucopterus (Say), will damage seedlings of summer grass crops of corn, sorghum and millet after infesting winter small grains (Metcalf et al. 1962). However, true chinch bugs were not collected in this study. Furthermore, soybean stands were 18-25% lower following canola than following small grains in all years. The cause of this stand reduction is not known. False chinch bugs were more numerous following canola than winter wheat or rye, but injury from chinch bugs does not explain the reduction in the number of soybean seedlings. Soybean stand losses most likely were caused by physical interference of the canola stubble with planter performance or possibly by undetermined chemical of biological parameters associated with canola stubble.
Except for false chinch bugs, previous winter crop had very little consistent effect on populations of insects on soybean or pearl millet. One exception was that thrips populations were greater on soybean seedlings following canola, but thrips usually are not economically important on soybean. These results show that continuous planting of a crop can enhance a host specific pest such as Hessian fly in wheat. Polyphagous pests which includes all insects pests collected in canola, soybean, and millet were not greatly affected by rotational sequence. When rotational effects occurred they were with seedling pests whose abundance was influenced by the immediate previous crop. Longer term rotational effects were not observed.
Pearl millet diseases. The $2 billion poultry industry in Georgia has eagerly accepted pearl millet as a new high-quality feed grain but current production is limited. The crop can be successfully grown in rotations with wheat or canola with no detrimental effects on diseases or yield of any of the crops. Some potential problems in pearl millet following canola, such as reduced seedling stands and increased populations of false chinch bugs, tend to be compensated for by reduced foliar disease and reduced stalk rot. Because yields of wheat, canola, and pearl millet are unaffected by rotations, the demands of the regional poultry industry can be addressed by introducing pearl millet into diversified agricultural production systems.
Canola and soybean diseases. The major diseases of canola have not developed as rapidly as expected. The other crops in the rotation have not had any measurable adverse influence on disease control in canola and no special disease control procedures will be needed for double cropped canola.
The expected soybean diseases have developed in the study. Foliar diseases have been erratic in incidence and have not been influenced by cropping history. Root and stem diseases, except stem canker, have a low incidence and have not increased significantly. Stem canker increased in incidence and severity each year and in 1997 was partially responsible for the very low seed yields. Apparent early season differences due to previous winter and summer crops did not last throughout the season and were not reflected in seed yields. To date there has been no clear influence of cropping history on stem canker or any other soybean disease. This indicates that the other crops in the rotations are compatible with soybeans and the addition of millet and canola to the doublecropping system will not adversely influence soybean disease control. Addition of these crops to the double cropping system will not require any major changes in soybean cultivar resistance or other disease control procedures.
Economic analyses. Comparisons of the rotation effects on returns were performed by using performance rates for the field operations recorded on specific crops from field operations at the branch experiment stations. The field operations were then costed using costs previously estimated for the equipment utilized to perform these operations. These costs were determined utilizing annual usage rates of the equipment at the branch experiment stations and repair costs relationships and equipment total use hours available estimated from ASAE EP 391.l (ASAE, 1986) and Rotig (1987).
Average prices received by Georgia farmers for the respective crops were used as the crop price for estimating revenue. Prices paid for plant nutrients and other chemicals by the branch experiment station were used for non-machinery inputs. Yields used in the crop and rotation budgeting were the means within statistical significant yield grouping, i.e., all yields of treatments not statistically significantly different were averaged and a single cost and return budget for the group was calculated. The return measure used was returns to land and management, i.e., land and management were not costed.
The returns by treatment and crop show that rye without any returns provides a costly winter cover crop and wheat significantly infected with take-all also provides costly segment of a crop rotation (Table 20). Higher weed control costs in the 1997 wheat crop and 20% lower yields removed the profitability of wheat compared to the 1995 year which had comparable wheat prices. Because of a crop freeze in the winter of 1995-1996, the returns for the 1996 canola crop were highly negative. Lower canola prices in 1997 made canola barely profitable in 1997. Drought caused lower millet yields in 1997 reduced the profitability of a low profitable crop. The yield reductions in soybeans following canola removes the profitability of soybeans in 1995 and 1996 and in 1997 doubled the loss caused by the drought. In 1997 soybeans in continuous wheat soybean rotations showed the same increase in negative returns as did soybeans after canola.
The rotation returns were computed under two assumptions; 1) take-all infection all years and 2) no take-all infection (Table 21). Under the assumption of take-all, all canola rotations but three show a positive return over three years, two (R-S, C-M, W-S and W-S, C-M, W-S) only had canola during the 1995-1996 winter in which canola froze and the third (W-M, R-S, C-S) had rye a negative income winter crop and canola in the third year which had low returns ($6.56/acre). Wheat rotations where take-all was severe were only profitable if canola was in the rotation. The wheat:millet rotations with severe take-all was the highest negative rotation ($283) with the rye:millet, wheat:soybean, and rye:soybean next (Table 21). With high take-all, the highest income rotations were wheat:canola and had a chance factor in them in that wheat was grown in the second year in which canola failed. If no or low take-all was present, the wheat and canola-wheat rotations were comparable if wheat happened to be the winter crop in the rotation during 1995-1996.
In the three year rotation sequences there were no economic benefits from rye as a winter cover crop. All rotations which included rye average negative incomes ranging from $-6/acre to $-150/acre with no take-all and from $-116/acre to $-206/acre with severe take-all.
Attempting to remove the "luck" results of the C-S, W-M, C-M, or the C-S, W-M, C-S rotations, the reverse of the rotations could be synthesized and an average of the reverse sequences (W-M, C-S, W-M, or W-M, C-M, W-M) compared with the results of the observed rotations. These estimated 3-year averages would be $79.58/acre for the C-S, W-M, C-M rotation and $104.87/acre for the C-S, W-M, C-S rotation. These computed averages should be compared with a synthesized rotation using gained knowledge of beneficial and elemental relationships and relative returns. The synthesized rotation would be C-M, W-S, C-M and the reverse W-S, C-M, W-S and the average 3-year income would be $156.10/acre. This synthesized rotation would have the take-all control by canola and would substitute the lower income of millet for the negative effect of canola and soybean.
Areas needing additional study
Brown, P.D., and Morra, M.J. 1997. Control of soil-borne plant pests using glucosinolate- containing plants. Pp. 167-231. In: D.L. Sparks (ed.). Advances in Agronomy Vol. 61. Academic Press.
Koan, M. and H. N. Padre. 1980. General sampling methods for above-ground populations of soybean arthropods. p. 30-60. In M. Koan & D.C. Herzog (ed.), Sampling methods in soybean Entomology. Springer-Verlag, N.Y.
Metcalf, C. L., W. P. Flint, and R. L. Metcalf. 1962. Destructive and Useful Insects. MacGraw-Hill, N. Y. p. 1087.
Rothrock, C.S., and Cunfer, B.M. 1991. Influence of small grain rotations on take-all in a subsequent wheat crop. Plant Dis. 75:1050-1052.
Rotig, C.A. 1987. A Standard Model for Repair Costs of Agricultural Machinery. Applied Engineering in Agriculture, pp. 3-9.
Shipton, P.J. 1972. Influence of stubble treatment and autumn application of nitrogen to stubbles on the subsequent incidence of take-all and eyespot. Plant Pathol. 21:147-155.