Project Overview
ANC95-032
Project Type: Research and Education
Funds awarded in 1995: $0.00
Projected End Date: 12/31/1997
Matching Non-Federal Funds: $63,091.00
ACE Funds: $40,000.00
Region: North Central
State: Wisconsin
Project Coordinator:
John Andrews
University of Wisconsin Madison
Annual Reports
Commodities
- Additional Plants: ornamentals, trees
Practices
- Natural Resources/Environment: biodiversity
- Pest Management: general pest management
Abstract:
Water extracts of slurries of spent mushroom substrate compost inhibit the apple scab pathogen Venturia inaequalis in the laboratory, and reduce severity of scab disease in the field. This project was an attempt to isolate the compound(s) responsible based on the polarity, charge, and size of the bioactive molecule(s). The methods used included extraction (partitioning) into nonpolar solvents at acidic, neutral, or basic pH; cation exchange column chromatography; paper electrophoresis; and ultrafiltration through membrane filters (microconcentrators) of known porosity or Sephadex G-10 and G-25 columns. Almost all of the activity was associated with polar (water-soluble) molecules and at least some of it was less than 3 kilodaltons in molecular weight.
Project objectives:
To continue determination of the pathogen-and disease-inhibitory principle(s) in the spent mushroom subtrate (SMS) extracts.
Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and should not be construed to represent any official USDA or U.S. Government determination or policy.