- Additional Plants: ornamentals, trees
- Natural Resources/Environment: biodiversity
- Pest Management: general pest management
Water extracts of slurries of spent mushroom substrate compost inhibit the apple scab pathogen Venturia inaequalis in the laboratory, and reduce severity of scab disease in the field. This project was an attempt to isolate the compound(s) responsible based on the polarity, charge, and size of the bioactive molecule(s). The methods used included extraction (partitioning) into nonpolar solvents at acidic, neutral, or basic pH; cation exchange column chromatography; paper electrophoresis; and ultrafiltration through membrane filters (microconcentrators) of known porosity or Sephadex G-10 and G-25 columns. Almost all of the activity was associated with polar (water-soluble) molecules and at least some of it was less than 3 kilodaltons in molecular weight.
To continue determination of the pathogen-and disease-inhibitory principle(s) in the spent mushroom subtrate (SMS) extracts.