Developing a System of Tissue Culture and Hydroponic Growing Medium for Hop Plant Production

Project Overview

Project Type: Farmer/Rancher
Funds awarded in 2016: $22,500.00
Projected End Date: 01/30/2018
Grant Recipient: Great Lakes Hydrogrow
Region: North Central
State: Michigan
Project Coordinator:
Tim Small
Great Lakes Hydrogrow Llc.

Annual Reports


  • Agronomic: hops


  • Crop Production: tissue analysis
  • Education and Training: participatory research
  • Farm Business Management: feasibility study
  • Pest Management: disease vectors
  • Production Systems: general crop production
  • Sustainable Communities: employment opportunities


    Upon approval of the grant, work began March 1, 2016.  Substantial research was initiated for the project.

    Upon determination that tissue culture labs are cost prohibitive, several tissue culture labs were contacted. One tissue culture lab was able to produce three varieties of Certified Virus and Disease free hop plants. 

    Great Lakes Hydrogrow was created for the research and development phase of Virus and Disease Free Hop Plant Production. Our website was produced to disseminate information about this project. 

    Project objectives:

    We began to study the process of tissue culture and what is required to be successful using this method. We studied the uses of tissue culture in hop plant production.  We reviewed the status of current research being completed.  We researched the feasibility of using tissue culture to produce disease free Hop plants.

    As a result of our research we located a project using tissue cultures for Hop plant production:

    'Addendum A'  The Elimination Of Viruses And Hop Latent Viroid From Hop In Poland.  '2005'

    ISHS Acta Horticulturae 668: I International Humulus Symposium



    Authors:   M. Grudzińska, E. Solarska
    Keywords:   virus and viroid elimination, in vitro culture, meristem tip, hop plants, PNRSV, HpMV, HLVd
    DOI:   10.17660/ActaHortic.2005.668.19
    Hops in Poland are commonly infected with Hop latent viroid (HLVd) and viruses: Prunus necrotic ringspot virus (PNRSV) and Hop mosaic virus (HpMV). Previous experiments were successful in obtaining virus-free hop plants by heat therapy method but appeared to be inefficient for HLVd elimination. In these studies virus and viroid free hop plants were obtained by meristem-tip culture. The meristem tips were excised from lateral shoot tips of naturally infected plants and placed in vitro. In vitro regeneration ability varied between 0 and 46% of regenerated explants depending upon cultivar, depending upon the time at which meristems tips were obtained. The regenerated plants were tested by ELISA for the presence of viruses and by RT-PCR for the presence of HLVd. Plants with no detectable viruses and HLVd were used for further propagation.
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    Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture or SARE.