Assessment of beneficial microorganisms: Trichoderma, Actinomycetes, and Bacillus in anaerobic soil disinfestation (ASD)

2014 Annual Report for GS14-128

Project Type: Graduate Student
Funds awarded in 2014: $10,993.00
Projected End Date: 12/31/2015
Grant Recipient: The University of Tennessee
Region: Southern
State: Tennessee
Graduate Student:
Major Professor:
Dr. David Butler
University of Tennessee

Assessment of beneficial microorganisms: Trichoderma, Actinomycetes, and Bacillus in anaerobic soil disinfestation (ASD)

Summary

In 2014, sclerotia of Sclerotium rolfsii were produced in the lab for use in pot studies associated with this project. Additionally, endophytic Trichoderma spp. were isolated from a field study and sequenced in order to better characterize beneficial microbes important to successful soil treatment by anaerobic soil disinfestation. Pot studies under environmentally-controlled conditions will be completed in 2015.

Objectives/Performance Targets

The main objectives in 2014 were to produce the large numbers of sclerotia of S. rolfsii necessary for pot studies in 2015, and to isolate, sequence, and culture beneficial endophytic Trichoderma spp. from field soil. Both of these objectives were met which will allow pot studies to meet main project objectives to proceed in 2015.

Accomplishments/Milestones

-Sclerotia of Sclerotium rolfsii were produced in the lab for use in pot studies (to be initiated in early 2015).
-Endophytic Trichoderma spp. were isolated from field soil, sequenced and identified (primarily T. asperellum) and cultured for use in pot studies (to be initiated in early 2015).

Impacts and Contributions/Outcomes

Nothing to report at this time.

Collaborators:

Dr. Bonnie Ownley

bownley@utk.edu
Professor
University of Tennessee, Dept. of Entomology and Plant Pathology
2505 E.J. Chapman Dr.
370 Plant Biotech Building
Knoxville, TN 37996
Office Phone: 8659740219
Utsala Shrestha

ushrest1@vols.utk.edu
Graduate Research Assistant
University of Tennessee, Plant Sciences
2431 Joe Johnson Dr
252 Ellington Plant Science Bldg
Knoxville, TN 37996
Office Phone: 8659748860