Assessment of beneficial microorganisms: Trichoderma, Actinomycetes, and Bacillus in anaerobic soil disinfestation (ASD)

Assessment of beneficial microorganisms: Trichoderma, Actinomycetes, and Bacillus in anaerobic soil disinfestation (ASD)

Summary

In 2014, sclerotia of Sclerotium rolfsii were produced in the lab for use in pot studies associated with this project. Additionally, endophytic Trichoderma spp. were isolated from a field study and sequenced in order to better characterize beneficial microbes important to successful soil treatment by anaerobic soil disinfestation. Pot studies under environmentally-controlled conditions will be completed in 2015.

Objectives/Performance Targets

The main objectives in 2014 were to produce the large numbers of sclerotia of S. rolfsii necessary for pot studies in 2015, and to isolate, sequence, and culture beneficial endophytic Trichoderma spp. from field soil. Both of these objectives were met which will allow pot studies to meet main project objectives to proceed in 2015.

Accomplishments/Milestones

-Sclerotia of Sclerotium rolfsii were produced in the lab for use in pot studies (to be initiated in early 2015).
-Endophytic Trichoderma spp. were isolated from field soil, sequenced and identified (primarily T. asperellum) and cultured for use in pot studies (to be initiated in early 2015).

Impacts and Contributions/Outcomes

Nothing to report at this time.

Collaborators: