- Animals: other
- Animal Products: other
- Education and Training: participatory research
- Natural Resources/Environment: biodiversity
- Soil Management: soil microbiology
The producers of vermicompost have long acknowledged the benefit of having a diverse and active soil microbial community. However, this diversity has yet to be properly quantified and is often still seen to be a black box. Next-generation DNA sequencing has allowed researchers to shed light into these black boxes and have led to large projects such as the Human Microbiome (Turnbaugh et al. 2007) and Earth Microbiome Projects (Gilbert et al. 2014). However, one of our most valuable resources in restoring soil and plant health, the microbial community of vermicompost, has been neglected and its producers left in the dark. The goals of this project are 1) develop a baseline microbial community analysis of vermicompost and teas from producers across the nation and 2) help this community better understand and quantify diversity in order to improve and increase adoption of their products. Questions such as “do inputs affect diversity,” “does vermicompost have a higher biological diversity than big box compost”, and “does education of producers and their clients help sales”? are among the many questions that can be addressed with this data. Physical and chemical analyses will also be used to evaluate vermicompost qualities to develop some understanding of how these familiar measurements relate to microbial communities. Communication through email and website will be crucial. We plan to present our results and conclusions at the 21st NC State Vermicomposting conference as well as publish in an open source journal. Education and outreach materials will be created and disseminated to producers.
Project objectives from proposal:
The overall objective of this project is to collect and analyze a large and diverse amount of vermicompost and vermicompost tea in order to enhance the knowledge of sustainable vermicomposting practices and to bring a new technology and understanding to compost producers. The first objective will be to collect samples nationally from vermicompost producers. We aim to collect 150-250 samples from as diverse group of producers as possible. Our second key objective is to successfully sequence received samples and have the vermicomposts analyzed conventionally using Seal of Testing Assurance Program (STA) methods. DNA extraction and sequencing preparation will be performed at the Colorado School of Mines saving significant sample analysis costs over a private lab. Compost STA properties will be analyzed by Midwest Labs.
Our third objective is to use bioinformatic tools to process the sequencing data for quality control and taxonomic assignment. By comparing the communities found in samples, the metadata from the vermicompost STA analysis, and producer answered questionnaires we can begin to answer key deliverable questions. These questions include: Is there a core microbial community found in all vermicomposting samples and why might they be universally present? Do composting inputs and techniques have an effect on overall diversity? Can a metric be developed based on this survey of samples to distinguish between different vermicomposts? How do the microbial communities of vermicompost change overtime? Can we also use this technology for pathogen tracking from manure inputs? We also want to compare vermicomposts to their tea derivatives and to big box vermicomposts and compost brands.
Our fourth objective is to educate the producers and the public about microbial soil diversity and it’s benefits to sustainable agriculture. This will be accomplished by virtual interaction, face to face discussion, publishing in an open source journal, and providing producers with a public outreach package.